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Polychlorinated biphenyls (PCBs) as initiating agents in hepatocellular carcinoma
Journal article   Open access   Peer reviewed

Polychlorinated biphenyls (PCBs) as initiating agents in hepatocellular carcinoma

Gabriele Ludewig and Larry W Robertson
Cancer letters, Vol.334(1), pp.46-55
06/28/2013
DOI: 10.1016/j.canlet.2012.11.041
PMCID: PMC3648605
PMID: 23211541
url
https://doi.org/10.1016/j.canlet.2012.11.041View
Published (Version of record) Open Access

Abstract

PCBs are carcinogens, but for many decades it was assumed that PCBs may not possess initiating activity. Initiation is a process that involves changes in the DNA sequence, often, but not exclusively produced through DNA adduction by a reactive compound or reactive oxygen species (ROS). DNA adducts can be detected by 32P-postlabeling, a method that Dr. Ramesh Gupta co-developed and refined. Today these types of assays together with other mechanistic studies provide convincing evidence that specific PCB congeners can be biotransformed to genotoxic and therefore potentially initiating metabolites. This review will provide an overview of our current knowledge of PCBs’ genotoxic potential and mechanism of action, emphasizing the contributions of Dr. Ramesh Gupta during his tenures at the Universities of Kentucky and Louisville.
Metabolic activation 32P-postlabeling Gene mutation PCB Genotoxicity

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