Journal article
Poly(diaminosulfide) Microparticle-Based Vaccine for Delivery of Leptospiral Antigens
Biomacromolecules, Vol.21(2), pp.534-544
02/10/2020
DOI: 10.1021/acs.biomac.9b01257
PMID: 31895553
Abstract
Leptospirosis is a debilitating infectious disease that detrimentally affects both animals and humans; therefore, disease prevention has become a high priority to avoid high incidence rates of disease in the herd and break the transmission cycle to humans. Thus, there remains an important unmet need for a prophylactic vaccine that can provide long-term immunity against leptospirosis in cattle. Herein, a novel vaccine formulation was developed where poly(diaminosulfide) polymer was employed to fabricate microparticles encapsulating the antigen of
serovar Hardjo strain HB15B203 (L203-PNSN). A prime-boost vaccination with a L203-PNSN microparticle formulation increased the population of L203-specific CD3
T cells and CD21
B cells to levels that were significantly higher than those of cattle vaccinated with L203-AlOH or the vehicle control (empty PNSN microparticles and blank AlOH). In addition, L203-PNSN was demonstrated to stimulate durable humoral immune responses as evidenced by the increases in the antibody serum titers following the vaccination. It was also found that cattle vaccinated with L203-PNSN produced higher macroscopic agglutinating titers than cattle in other groups. Thus, it can be concluded that L203-PNSN is a novel first-in-class microparticle-based
vaccine that represents a powerful platform with the potential to serve as a prophylactic vaccine against leptospiral infection in cattle.
Details
- Title: Subtitle
- Poly(diaminosulfide) Microparticle-Based Vaccine for Delivery of Leptospiral Antigens
- Creators
- Emad I WafaJennifer H Wilson-Welder - Infectious Bacterial Disease Research Unit, National Animal Disease Center, Agriculture Research Service , United States Department of Agriculture , Ames , Iowa 50010 , United StatesRichard L Hornsby - Infectious Bacterial Disease Research Unit, National Animal Disease Center, Agriculture Research Service , United States Department of Agriculture , Ames , Iowa 50010 , United StatesJarlath E Nally - Infectious Bacterial Disease Research Unit, National Animal Disease Center, Agriculture Research Service , United States Department of Agriculture , Ames , Iowa 50010 , United StatesSean M GearyNed B BowdenAliasger K Salem
- Resource Type
- Journal article
- Publication Details
- Biomacromolecules, Vol.21(2), pp.534-544
- DOI
- 10.1021/acs.biomac.9b01257
- PMID
- 31895553
- NLM abbreviation
- Biomacromolecules
- ISSN
- 1525-7797
- eISSN
- 1526-4602
- Grant note
- P30 CA086862 / NCI NIH HHS
- Language
- English
- Date published
- 02/10/2020
- Academic Unit
- Roy J. Carver Department of Biomedical Engineering; Pharmaceutical Sciences and Experimental Therapeutics; Craniofacial Anomalies Research Center; Chemistry; Dental Research; Chemical and Biochemical Engineering
- Record Identifier
- 9984216691802771
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