Journal article
Poly(galactaramidoamine) is an efficient cationic polymeric non-viral vector with low cytotoxicity for transfecting human embryonic kidney (HEK293) and murine macrophage (RAW264.7) cells
Pharmaceutical Development and Technology, Vol.18(5), pp.1255-1258
10/01/2013
DOI: 10.3109/10837450.2011.649856
PMCID: PMC3638068
PMID: 22235917
Abstract
Poly(galactaramidoamine) (PGAA) is a cationic co-polymer of dimethyl-meso-galactarate and pentaethylenehexamine. PGAA electrostatically complexes with plasmid DNA (pDNA) to form nano-sized particles. In this study, we show that PGAA-pDNA polyplexes generate high transfection efficiencies in human embryonic kidney (HEK293) and murine macrophage-like (RAW264.7) cell lines. PGAA-pDNA mediated transfection is a function of the amine:phosphate (N/P) ratio at which the polyplexes are prepared. The maximum expression of luciferase was obtained using polyplexes prepared at an N/P ratio of 40. Polyplexes prepared at increasing N/P ratios did not significantly increase in size but did result in decreasing luciferase expression. Cellular toxicity increased as the N/P ratios at which the polyplexes were prepared increased.
Details
- Title: Subtitle
- Poly(galactaramidoamine) is an efficient cationic polymeric non-viral vector with low cytotoxicity for transfecting human embryonic kidney (HEK293) and murine macrophage (RAW264.7) cells
- Creators
- Amaraporn Wongrakpanich - Department of Pharmaceutical Sciences and Experimental Therapeutics, College of Pharmacy, University of IowaVijaya B Joshi - Department of Pharmaceutical Sciences and Experimental Therapeutics, College of Pharmacy, University of IowaAliasger K Salem - Department of Pharmaceutical Sciences and Experimental Therapeutics, College of Pharmacy, University of Iowa
- Resource Type
- Journal article
- Publication Details
- Pharmaceutical Development and Technology, Vol.18(5), pp.1255-1258
- DOI
- 10.3109/10837450.2011.649856
- PMID
- 22235917
- PMCID
- PMC3638068
- NLM abbreviation
- Pharm Dev Technol
- ISSN
- 1083-7450
- eISSN
- 1097-9867
- Publisher
- Taylor & Francis
- Language
- English
- Date published
- 10/01/2013
- Academic Unit
- Roy J. Carver Department of Biomedical Engineering; Pharmaceutical Sciences and Experimental Therapeutics; Craniofacial Anomalies Research Center; Dental Research; Chemical and Biochemical Engineering
- Record Identifier
- 9983986547702771
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