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Polymer Coated Echogenic Lipid Nanoparticles with Dual Release Triggers
Journal article   Open access   Peer reviewed

Polymer Coated Echogenic Lipid Nanoparticles with Dual Release Triggers

Rahul Nahire, Manas K. Haldar, Shirshendu Paul, Anaas Mergoum, Avinash H. Ambre, Kalpana S. Katti, Kara N. Gange, D. K. Srivastava, Kausik Sarkar and Sanku Mallik
Biomacromolecules, Vol.14(3), pp.841-853
02/20/2013
DOI: 10.1021/bm301894z
PMCID: PMC3595326
PMID: 23394107
url
https://www.ncbi.nlm.nih.gov/pmc/articles/3595326View
Open Access

Abstract

Although lipid nanoparticles are promising drug delivery vehicles, passive release of encapsulated contents at the target site is often slow. Herein, we report contents release from targeted, polymer coated, echogenic lipid nanoparticles in the cell cytoplasm by redox trigger and simultaneously enhanced by diagnostic frequency ultrasound. The lipid nanoparticles were polymerized on the external leaflet using a disulfide cross-linker. In the presence of cytosolic concentrations of glutathione, the lipid nanoparticles released 76% of encapsulated contents. Plasma concentrations of glutathione failed to release the encapsulated contents. Application of 3 MHz ultrasound for 2 minutes simultaneously with the reducing agent enhanced the release to 96%. Folic acid conjugated, doxorubicin loaded nanoparticles showed enhanced uptake and higher cytotoxicity in cancer cells overexpressing the folate receptor (compared to the control). With further developments, these lipid nanoparticles have the potential to be used as multimodal nanocarriers for simultaneous targeted drug delivery and ultrasound imaging.
Echogenic liposomes Lipid nanoparticles Liposomes Redox triggered release Targeted drug delivery Ultrasound

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