Journal article
Polymersomes Decorated with the SARS-CoV-2 Spike Protein Receptor-Binding Domain Elicit Robust Humoral and Cellular Immunity
ACS central science, Vol.7(8), pp.1368-1380
08/25/2021
DOI: 10.1021/acscentsci.1c00596
PMCID: PMC8315245
PMID: 34466656
Abstract
The COVID-19 pandemic underscores the need for rapid, safe, and effective vaccines. In contrast to some traditional vaccines, nanoparticle-based subunit vaccines are particularly efficient in trafficking antigens to lymph nodes, where they induce potent immune cell activation. Here, we developed a strategy to decorate the surface of oxidation-sensitive polymersomes with multiple copies of the SARS-CoV-2 spike protein receptor-binding domain (RBD) to mimic the physical form of a virus particle. We evaluated the vaccination efficacy of these surface-decorated polymersomes (RBDsurf) in mice compared to RBD-encapsulated polymersomes (RBDencap) and unformulated RBD (RBDfree), using monophosphoryl-lipid-A-encapsulated polymersomes (MPLA PS) as an adjuvant. While all three groups produced high titers of RBD-specific IgG, only RBDsurf elicited a neutralizing antibody response to SARS-CoV-2 comparable to that of human convalescent plasma. Moreover, RBDsurf was the only group to significantly increase the proportion of RBD-specific germinal center B cells in the vaccination-site draining lymph nodes. Both RBDsurf and RBDencap drove similarly robust CD4(+) and CD8(+) T cell responses that produced multiple Th1-type cytokines. We conclude that a multivalent surface display of spike RBD on polymersomes promotes a potent neutralizing antibody response to SARS-CoV-2, while both antigen formulations promote robust T cell immunity.
Details
- Title: Subtitle
- Polymersomes Decorated with the SARS-CoV-2 Spike Protein Receptor-Binding Domain Elicit Robust Humoral and Cellular Immunity
- Creators
- Lisa R. Volpatti - University of ChicagoRachel P. Wallace - University of ChicagoShijie Cao - University of ChicagoMichal M. Raczy - University of ChicagoRuyi Wang - University of ChicagoLaura T. Gray - University of ChicagoAaron T. Alpar - University of ChicagoPriscilla S. Briquez - University of ChicagoNikolaos Mitrousis - University of ChicagoTiffany M. Marchell - University of ChicagoMaria Stella Sasso - University of ChicagoMindy Nguyen - University of ChicagoAslan Mansurov - University of ChicagoErica Budina - University of ChicagoAni Solanki - University of ChicagoElyse A. Watkins - University of ChicagoMathew R. Schnorenberg - University of ChicagoAndrew C. Tremain - University of ChicagoJoseph W. Reda - University of ChicagoVlad Nicolaescu - University of ChicagoKevin Furlong - University of ChicagoSteve Dvorkin - University of ChicagoShann S. Yu - University of ChicagoBalaji Manicassamy - University of IowaJames L. LaBelle - Comer Children's HospitalMatthew Tirrell - Argonne National LaboratoryGlenn Randall - University of ChicagoMarcin Kwissa - University of ChicagoMelody A. Swartz - University of ChicagoJeffrey A. Hubbell - University of Chicago
- Resource Type
- Journal article
- Publication Details
- ACS central science, Vol.7(8), pp.1368-1380
- DOI
- 10.1021/acscentsci.1c00596
- PMID
- 34466656
- PMCID
- PMC8315245
- NLM abbreviation
- ACS Cent Sci
- ISSN
- 2374-7943
- eISSN
- 2374-7951
- Publisher
- Amer Chemical Soc
- Number of pages
- 13
- Grant note
- HHSN272201400008C; P30CA014599 / NIH NIAID; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Institute of Allergy & Infectious Diseases (NIAID) Chicago Immunoengineering Innovation Center of the University of Chicago CR002 / Chicago Biomedical Consortium COVID-19 Response Award T32-HL007605 / NIH NHLBI; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Heart Lung & Blood Institute (NHLBI) 201910MFE-430736-73744 / Canadian Institutes of Health Research; Canadian Institutes of Health Research (CIHR) T32-GM007281 / NIH NIGMS; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Institute of General Medical Sciences (NIGMS) CHE-1048528; SCR_019198; SCR_017916 / Soft Matter Characterization Facility University of Chicago Comprehensive Cancer Center (Sigal Fellowship in ImmunoOncology) F30-CA221250 / NIH NCI; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Cancer Institute (NCI)
- Language
- English
- Date published
- 08/25/2021
- Academic Unit
- Microbiology and Immunology
- Record Identifier
- 9984297431702771
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