Journal article
Polymorphonuclear neutrophils delay corneal epithelial wound healing in vitro
Investigative ophthalmology & visual science, Vol.25(10), pp.1217-1220
10/1984
PMID: 6480296
Abstract
Using an in vitro system for measuring epithelial wound healing, we studied the effect of polymorphonuclear neutrophils (PMNs) and PMN lysate on rat corneal epithelial wound healing. After 22 hr of organ culture, epithelial defects that were originally 3 mm in diameter (7.06 mm2) measured 0.41 mm2 (range, 0.17-0.72 mm2) in control rat corneas, 2.11 mm2 (range, 0.81-3.91 mm2) in corneas incubated in medium containing 5 X 10(6) PMN/ml, and 2.49 mm2 (range, 2.21-2.76 mm2) in corneas incubated with lysate obtained from an equivalent number of PMNs. Scanning and transmission electron microscopy showed similar morphology in the three groups. PMNs selectively adhered to the leading edge of the wound in corneas incubated with PMNs. These data indicate that PMNs and PMN lysate significantly slow corneal epithelial wound healing in vitro.
Details
- Title: Subtitle
- Polymorphonuclear neutrophils delay corneal epithelial wound healing in vitro
- Creators
- Michael D WagonerKenneth R KenyonIlene K GipsonLaila A HanninenWen L Seng
- Resource Type
- Journal article
- Publication Details
- Investigative ophthalmology & visual science, Vol.25(10), pp.1217-1220
- PMID
- 6480296
- NLM abbreviation
- Invest Ophthalmol Vis Sci
- ISSN
- 0146-0404
- eISSN
- 1552-5783
- Publisher
- United States
- Grant note
- EY-03967 / NEI NIH HHS EY-05718 / NEI NIH HHS EY-03306 / NEI NIH HHS
- Language
- English
- Date published
- 10/1984
- Academic Unit
- Ophthalmology and Visual Sciences
- Record Identifier
- 9983980297502771
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