Journal article
Polysaccharide K induces Mn superoxide dismutase (Mn-SOD) in tumor tissues and inhibits malignant progression of QR-32 tumor cells: possible roles of interferon α, tumor necrosis factor α and transforming growth factor β in Mn-SOD induction by polysaccharide K
Cancer Immunology, Immunotherapy, Vol.46(6), pp.338-344
08/1998
DOI: 10.1007/s002620050495
PMCID: PMC11037325
PMID: 9756418
Abstract
Previously we reported the malignant progression of QR-32, a regressor-type tumor clone, following co-implantation with foreign bodies (gelatin sponge or plastic plate) in normal syngeneic C57BL/6 mice. We also reported that the progression of QR-32 cells by a gelatin sponge was significantly inhibited in the mice administered polysaccharide K (PSK) and that PSK induced an increase of radical scavengers, especially manganese superoxide dismutase (Mn-SOD), locally at the site of tumor tissues. In this study, to reveal the possible mechanism by which PSK induced Mn-SOD in the tumor tissues, we examined the mRNA expression and protein levels of inflammatory cytokines in the tissues. We found that mRNAs of tumor necrosis factor α (TNFα) and interleukin-1α (IL-1α) were considerably expressed in both PSK-treated and phosphate-buffered-saline-treated tumors, and that the mRNA expression and protein level of interferon γ (IFNγ) increased in the tumor tissues treated with PSK. In vitro treatment of QR-32 cells with IFNγ did not significantly increase the production of Mn-SOD; however, the combination of IFNγ with TNFα increased the Mn-SOD production more effectively than did any of the cytokines used singly. Furthermore, we observed the down-regulation of the mRNA expression and protein level of transforming growth factor β (TGFβ) in the tumor tissues treated with PSK, and that in vitro treatment of QR-32 cells with TGFβ decreased the production of Mn-SOD. These results suggest that PSK suppresses the progression of QR-32 cells by increasing Mn-SOD via the modulation of inflammatory cytokines; that is, by decreasing TGF-β and increasing IFN-γ.
Details
- Title: Subtitle
- Polysaccharide K induces Mn superoxide dismutase (Mn-SOD) in tumor tissues and inhibits malignant progression of QR-32 tumor cells: possible roles of interferon α, tumor necrosis factor α and transforming growth factor β in Mn-SOD induction by polysaccharide K
- Creators
- Hasem Habelhah - Laboratory of Pathology, Cancer Institute, Hokkaido University School of Medicine, Kita-15, Nishi-7, Kita-ku, Sapporo, 060-8638, Japan Tel.: +81–11–706–5070; Fax: +81–11–706–7826 JPFutoshi Okada - Laboratory of Pathology, Cancer Institute, Hokkaido University School of Medicine, Kita-15, Nishi-7, Kita-ku, Sapporo, 060-8638, Japan Tel.: +81–11–706–5070; Fax: +81–11–706–7826 JPKazumoto Nakai - Laboratory of Pathology, Cancer Institute, Hokkaido University School of Medicine, Kita-15, Nishi-7, Kita-ku, Sapporo, 060-8638, Japan Tel.: +81–11–706–5070; Fax: +81–11–706–7826 JPSung Ki Choi - Laboratory of Pathology, Cancer Institute, Hokkaido University School of Medicine, Kita-15, Nishi-7, Kita-ku, Sapporo, 060-8638, Japan Tel.: +81–11–706–5070; Fax: +81–11–706–7826 JPJun-ichi Hamada - Division of Cell Biology, Cancer Institute, Hokkaido University School of Medicine, Kita-15, Nishi-7, Kita-ku, Sapporo, 060-8638, Japan JPMasanobu Kobayashi - Laboratory of Pathology, Cancer Institute, Hokkaido University School of Medicine, Kita-15, Nishi-7, Kita-ku, Sapporo, 060-8638, Japan Tel.: +81–11–706–5070; Fax: +81–11–706–7826 JPM Hosokawa - Laboratory of Pathology, Cancer Institute, Hokkaido University School of Medicine, Kita-15, Nishi-7, Kita-ku, Sapporo, 060-8638, Japan Tel.: +81–11–706–5070; Fax: +81–11–706–7826 JP
- Resource Type
- Journal article
- Publication Details
- Cancer Immunology, Immunotherapy, Vol.46(6), pp.338-344
- DOI
- 10.1007/s002620050495
- PMID
- 9756418
- PMCID
- PMC11037325
- NLM abbreviation
- Cancer Immunol Immunother
- ISSN
- 0340-7004
- eISSN
- 1432-0851
- Publisher
- Springer-Verlag; Berlin/Heidelberg
- Language
- English
- Date published
- 08/1998
- Academic Unit
- Pathology
- Record Identifier
- 9984046914702771
Metrics
11 Record Views