Journal article
Post-Weaning Social Isolation Alters Puberty Onset by Suppressing Electrical Activity of Arcuate Kisspeptin Neurons
Neuroendocrinology, Vol.114(5), pp.439-452
01/25/2024
DOI: 10.1159/000535721
PMCID: PMC11098025
PMID: 38271999
Abstract
Post-weaning social isolation (PWSI) in rodents is an advanced psychosocial stress model in early life. Some psychosocial stress, such as restrain and isolation, disrupt reproductive physiology in young and adult period. Mechanisms of early life stress (ELS) effects on central regulation of reproduction needs to be elucidated. We have investigated the effects of PWSI on function of Arcuate Kisspeptin (ARCKISS1) neurons by using electrophysiological techniques combining with monitoring of puberty onset and estrous cycle in male and female Kiss1-Cre mice.
Female mice were monitored for puberty onset with vaginal opening examination during social isolation. After isolation, estrous cycle of female mice was monitored with vaginal cytology. Anxiety-like behavior of mice was determined by elevated plus maze test. Effects of PWSI on electrophysiology of ARCKISS1 neurons were investigated by patch clamp method after intracranial injection of AAV-GFP virus into arcuate nucleus of Kiss1-Cre mice after isolation period.
We found that both male and female isolated mice showed anxiety-like behavior. PWSI caused delay in vaginal opening and extension in estrous cycle length. Spontaneous firing rate of ARCKISS1 neurons were significantly lower in the isolated male and female mice. The peak amplitude of inhibitory post-synaptic currents to ARCKISS1 neurons was higher in the isolated mice while frequency of excitatory post-synaptic currents was higher in group housed mice.
These findings demonstrate that PWSI alters pre- and post- pubertal reproductive physiology through metabolic and electrophysiological pathways.
Details
- Title: Subtitle
- Post-Weaning Social Isolation Alters Puberty Onset by Suppressing Electrical Activity of Arcuate Kisspeptin Neurons
- Creators
- Sami AgusYavuz YavuzDeniz AtasoyBayram Yilmaz
- Resource Type
- Journal article
- Publication Details
- Neuroendocrinology, Vol.114(5), pp.439-452
- DOI
- 10.1159/000535721
- PMID
- 38271999
- PMCID
- PMC11098025
- eISSN
- 1423-0194
- Language
- English
- Electronic publication date
- 01/25/2024
- Academic Unit
- Iowa Neuroscience Institute; Fraternal Order of Eagles Diabetes Research Center; Neuroscience and Pharmacology
- Record Identifier
- 9984548467102771
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