Journal article
Post-transcriptional regulation of syndecan-1 expression by cAMP in peritoneal macrophages
The Journal of cell biology, Vol.122(4), pp.941-950
08/1993
DOI: 10.1083/jcb.122.4.941
PMID: 8394371
Abstract
Syndecan-1 is a cell surface heparan sulfate proteoglycan that is proposed to serve in cell-cell adhesion, cell-matrix anchorage, and growth factor signaling. Its expression is temporally and spatially regulated during epithelial-mesenchymal interactions in many developing tissues. In some cases, this regulation appears to be achieved at the level of transcription. However, induction of syndecan-1 expression in the embryonic kidney mesenchyme is suggested to occur at the level of mRNA translation (Vainio, S., M. Jalkanen, M. Bernfield, and L. Saxén. 1992. Dev. Biol. 152:221-232). To identify a system in which the regulatory mechanisms controlling syndecan-1 expression can be studied, cells of the monocyte-macrophage lineage, which regulate the expression of many cell surface receptors, were screened for syndecan-1 expression. The syndecan-1 gene is active in blood monocytes as well as resident and thioglycollate-elicited mouse peritoneal macrophages, but expression of the proteoglycan is regulated at two levels. First, elicited macrophages accumulate nine-fold more syndecan-1 mRNA than do resident macrophages or circulating blood monocytes. Another member of the syndecan family of proteoglycans, syndecan-4, shows a distinct pattern of expression, suggesting that this regulation is specific for syndecan-1. Second, utilization of the mRNA for syndecan-1 production encounters a post-transcriptional block in the elicited macrophages that can be overcome by triggering agents such as E-type prostaglandins or dibutyryl cAMP, which raise intracellular cAMP levels. Dibutyryl cAMP does not induce syndecan-1 expression in resident peritoneal macrophages, which lack a pool of stored mRNA. This suggests that this agent promotes the post-transcriptional utilization of stored syndecan-1 mRNA. The induced proteoglycan appears at the cell surface as a integral of 100-kD heparan sulfate-rich isoform of syndecan-1. This suggests that a cAMP-dependent post-transcriptional control mechanism may be present in a variety of tissues when syndecan-1 expression is regulated.
Details
- Title: Subtitle
- Post-transcriptional regulation of syndecan-1 expression by cAMP in peritoneal macrophages
- Creators
- Charles Yeaman - Program in Cell and Molecular Biology, University of Wisconsin-Madison 53706-1532Alan C Rapraeger
- Resource Type
- Journal article
- Publication Details
- The Journal of cell biology, Vol.122(4), pp.941-950
- Publisher
- United States
- DOI
- 10.1083/jcb.122.4.941
- PMID
- 8394371
- ISSN
- 0021-9525
- eISSN
- 1540-8140
- Grant note
- HD21881 / NICHD NIH HHS 5 T32 GM07215 / NIGMS NIH HHS
- Language
- English
- Date published
- 08/1993
- Academic Unit
- Anatomy and Cell Biology; Internal Medicine
- Record Identifier
- 9984025691302771
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