Postnatal expression of neurotrophic factors accessible to spiral ganglion neurons in the auditory system of adult hearing and deafened rats

Erin M Bailey; Steven H Green

doi:10.1523/JNEUROSCI.1014-14.2014

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Postnatal expression of neurotrophic factors accessible to spiral ganglion neurons in the auditory system of adult hearing and deafened rats

Journal article

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Postnatal expression of neurotrophic factors accessible to spiral ganglion neurons in the auditory system of adult hearing and deafened rats

Erin M Bailey and Steven H Green

The Journal of neuroscience, Vol.34(39), pp.13110-13126

09/24/2014

DOI: 10.1523/JNEUROSCI.1014-14.2014

PMCID: PMC4172804

PMID: 25253857

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https://doi.org/10.1523/JNEUROSCI.1014-14.2014View

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Abstract

Spiral ganglion neurons (SGNs) receive input from cochlear hair cells and project from the cochlea to the cochlear nucleus. After destruction of hair cells with aminoglycoside antibiotics or noise, SGNs gradually die. It has been assumed that SGN death is attributable to loss of neurotrophic factors (NTFs) derived from hair cells or supporting cells in the organ of Corti (OC). We used quantitative PCR (qPCR) to assay NTF expression-neurotrophin-3 (NT-3), BDNF, GDNF, neurturin, artemin, and CNTF-in the OC and cochlear nucleus at various ages from postnatal day 0 (P0) to P90 in control hearing and neonatally deafened rats. NT-3, neurturin, and CNTF were most abundant in the postnatal hearing OC; CNTF and neurturin most abundant in the cochlear nucleus. In the OC, NT-3 and CNTF showed a postnatal increase in expression approximately concomitant with hearing onset. In rats deafened by daily kanamycin injections (from P8 to P16), surviving inner hair cells were evident at P16 but absent by P19, with most postsynaptic boutons lost before P16. NT-3 and CNTF, which normally increase postnatally, had significantly reduced expression in the OC of deafened rats, although CNTF was expressed throughout the time that SGNs were dying. In contrast, neurturin expression was constant, unaffected by deafening or by age. CNTF and neurturin expression in the cochlear nucleus was unaffected by deafening or age. Thus, NTFs other than NT-3 are available to SGNs even as they are dying after deafening, apparently conflicting with the hypothesis that SGN death is attributable to lack of NTFs.

Animals

Cochlear Nucleus - cytology

Cochlear Nucleus - growth & development

Cochlear Nucleus - metabolism

Deafness - chemically induced

Deafness - metabolism

Gene Expression Regulation, Developmental

Hair Cells, Auditory - metabolism

Kanamycin - toxicity

Nerve Growth Factors - genetics

Nerve Growth Factors - metabolism

Organ Specificity

Presynaptic Terminals - metabolism

Rats

Rats, Sprague-Dawley

Spiral Ganglion - cytology

Spiral Ganglion - growth & development

Spiral Ganglion - metabolism

Details

Title: Subtitle: Postnatal expression of neurotrophic factors accessible to spiral ganglion neurons in the auditory system of adult hearing and deafened rats
Creators: Erin M Bailey - Departments of Biology and
Steven H Green - Departments of Biology and Otolaryngology, University of Iowa, Iowa City, Iowa 52242 steven-green@uiowa.edu
Resource Type: Journal article
Publication Details: The Journal of neuroscience, Vol.34(39), pp.13110-13126
DOI: 10.1523/JNEUROSCI.1014-14.2014
PMID: 25253857
PMCID: PMC4172804
ISSN: 0270-6474
eISSN: 1529-2401
Grant note: F31 DC011680 / NIDCD NIH HHS R01 DC02961 / NIDCD NIH HHS DC009405 / NIDCD NIH HHS P30 DC010362 / NIDCD NIH HHS R01 DC002961 / NIDCD NIH HHS R01 DC009405 / NIDCD NIH HHS
Language: English
Date published: 09/24/2014
Academic Unit: Biology; Otolaryngology
Record Identifier: 9984217428302771

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