Postprandial sleep mechanics in Drosophila

Keith R Murphy; Sonali A Deshpande; Maria E Yurgel; James P Quinn; Jennifer L Weissbach; Alex C Keene; Ken Dawson-Scully; Robert Huber; Seth M Tomchik; William W Ja

doi:10.7554/eLife.19334

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Postprandial sleep mechanics in Drosophila

Journal article

Open access

Peer reviewed

Postprandial sleep mechanics in Drosophila

Keith R Murphy, Sonali A Deshpande, Maria E Yurgel, James P Quinn, Jennifer L Weissbach, Alex C Keene, Ken Dawson-Scully, Robert Huber, Seth M Tomchik and William W Ja

eLife, Vol.5(2016), e19334

11/22/2016

DOI: 10.7554/eLife.19334

PMCID: PMC5119887

PMID: 27873574

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https://doi.org/10.7554/eLife.19334View

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Abstract

Food consumption is thought to induce sleepiness. However, little is known about how postprandial sleep is regulated. Here, we simultaneously measured sleep and food intake of individual flies and found a transient rise in sleep following meals. Depending on the amount consumed, the effect ranged from slightly arousing to strongly sleep inducing. Postprandial sleep was positively correlated with ingested volume, protein, and salt-but not sucrose-revealing meal property-specific regulation. Silencing of leucokinin receptor (Lkr) neurons specifically reduced sleep induced by protein consumption. Thermogenetic stimulation of leucokinin (Lk) neurons decreased whereas Lk downregulation by RNAi increased postprandial sleep, suggestive of an inhibitory connection in the Lk-Lkr circuit. We further identified a subset of non-leucokininergic cells proximal to Lkr neurons that rhythmically increased postprandial sleep when silenced, suggesting that these cells are cyclically gated inhibitory inputs to Lkr neurons. Together, these findings reveal the dynamic nature of postprandial sleep.

Animals

Drosophila - physiology

Eating

Neurons - physiology

Postprandial Period

Sleep

Details

Title: Subtitle: Postprandial sleep mechanics in Drosophila
Creators: Keith R Murphy - Scripps Research Institute
Sonali A Deshpande - Scripps Research Institute
Maria E Yurgel - Florida Atlantic University
James P Quinn - Scripps Research Institute
Jennifer L Weissbach - Scripps Research Institute
Alex C Keene - Florida Atlantic University
Ken Dawson-Scully - Florida Atlantic University
Robert Huber - Bowling Green State University
Seth M Tomchik - Scripps Research Institute
William W Ja - Scripps Research Institute
Resource Type: Journal article
Publication Details: eLife, Vol.5(2016), e19334
DOI: 10.7554/eLife.19334
PMID: 27873574
PMCID: PMC5119887
NLM abbreviation: Elife
ISSN: 2050-084X
eISSN: 2050-084X
Grant note: R21 DK092735 / NIDDK NIH HHS R01 NS085152 / NINDS NIH HHS
Language: English
Date published: 11/22/2016
Academic Unit: Iowa Neuroscience Institute; Neuroscience and Pharmacology
Record Identifier: 9984303851302771

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