Posttraining optogenetic manipulations of basolateral amygdala activity modulate consolidation of inhibitory avoidance memory in rats

Mary L Huff; Rachel L Miller; Karl Deisseroth; David E Moorman; Ryan T LaLumiere

doi:10.1073/pnas.1219593110

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Posttraining optogenetic manipulations of basolateral amygdala activity modulate consolidation of inhibitory avoidance memory in rats

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Posttraining optogenetic manipulations of basolateral amygdala activity modulate consolidation of inhibitory avoidance memory in rats

Mary L Huff, Rachel L Miller, Karl Deisseroth, David E Moorman and Ryan T LaLumiere

Proceedings of the National Academy of Sciences - PNAS, Vol.110(9), pp.3597-3602

02/26/2013

DOI: 10.1073/pnas.1219593110

PMCID: PMC3587230

PMID: 23401523

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Abstract

Memory consolidation studies, including those examining the role of the basolateral amygdala (BLA), have traditionally used techniques limited in their temporal and spatial precision. The development of optogenetics provides increased precision in the control of neuronal activity that can be used to address the temporal nature of the modulation of memory consolidation. The present experiments, therefore, investigated whether optogenetically stimulating and inhibiting BLA activity immediately after training on an inhibitory avoidance task enhances and impairs retention, respectively. The BLA of male Sprague–Dawley rats was transduced to express either ChR2(E123A) or archaerhodopsin-3 from the Halorubrum sodomense strain TP009 (ArchT). Immediately after inhibitory avoidance training, rats received optical stimulation or inhibition of the BLA, and 2 d later, rats’ retention was tested. Stimulation of ChR2(E123A)-expressing neurons in the BLA using trains of 40-Hz light pulses enhanced retention, consistent with recording studies suggesting the importance of BLA activity at this frequency. Light pulses alone given to control rats had no effect on retention. Inhibition of ArchT-expressing neurons in the BLA for 15 min, but not 1 min, significantly impaired retention. Again, illumination alone given to control rats had no effect on retention, and BLA inhibition 3 h after training had no effect. These findings provide critical evidence of the importance of specific frequency patterns of activity in the BLA during consolidation and indicate that optogenetic manipulations can be used to alter activity after a learning event to investigate the processes underlying memory consolidation.

Biological Sciences

Details

Title: Subtitle: Posttraining optogenetic manipulations of basolateral amygdala activity modulate consolidation of inhibitory avoidance memory in rats
Creators: Mary L Huff - Department of Psychology
Rachel L Miller - Department of Psychology
Karl Deisseroth - Departments of
David E Moorman - Department of Neurosciences
Ryan T LaLumiere - Department of Psychology
Resource Type: Journal article
Publication Details: Proceedings of the National Academy of Sciences - PNAS, Vol.110(9), pp.3597-3602
DOI: 10.1073/pnas.1219593110
PMID: 23401523
PMCID: PMC3587230
NLM abbreviation: Proc Natl Acad Sci U S A
ISSN: 0027-8424
eISSN: 1091-6490
Publisher: National Academy of Sciences
Alternative title: Optogenetic modulation of memory consolidation
Language: English
Date published: 02/26/2013
Academic Unit: Psychological and Brain Sciences; Iowa Neuroscience Institute
Record Identifier: 9984070269702771

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