Journal article
Potassium channel KCNJ15 is required for histamine-stimulated gastric acid secretion
American Journal of Physiology: Cell Physiology, Vol.309(4), pp.C264-C270
08/15/2015
DOI: 10.1152/ajpcell.00012.2015
PMID: 26108660
Abstract
Gastric acid secretion is mediated by the K(+)-dependent proton pump (H(+),K(+)-ATPase), which requires a continuous supply of K(+) at the luminal side of the apical membrane. Several K(+) channels are implicated in gastric acid secretion. However, the identity of the K(+) channel(s) responsible for apical K(+) supply is still elusive. Our previous studies have shown the translocation of KCNJ15 from cytoplasmic vesicles to the apical membrane on stimulation, indicating its involvement in gastric acid secretion. In this study, the stimulation associated trafficking of KCNJ15 was observed in a more native context with a live cell imaging system. KCNJ15 molecules in resting live cells were scattered in cytoplasm but exhibited apical localization after stimulation. Furthermore, knocking down KCNJ15 expression with a short hairpin RNA adenoviral construct abolished histamine-stimulated acid secretion in rabbit primary parietal cells. Moreover, KCNJ15, like H(+),K(+)-ATPase, was detected in all of the parietal cells by immunofluorescence staining, whereas only about half of the parietal cells were positive for KCNQ1 under the same condition. Consistently, the endogenous protein levels of KCNJ15, analyzed by Western blotting, were higher than those of KCNQ1 in the gastric mucosa of human, mouse, and rabbit. These results provide evidence for a major role of KCNJ15 in apical K(+) supply during stimulated acid secretion.
Details
- Title: Subtitle
- Potassium channel KCNJ15 is required for histamine-stimulated gastric acid secretion
- Creators
- Jianye Yuan - Longhua Hospital Shanghai University of Traditional Chinese MedicineWensheng Liu - University at Buffalo, State University of New YorkSerhan Karvar - Medical University of South CarolinaSusan S Baker - University at Buffalo, State University of New YorkWenjun He - University at Buffalo, State University of New YorkRobert D Baker - University at Buffalo, State University of New YorkGuang Ji - Shanghai University of Traditional Chinese MedicineJianqun Xie - Longhua Hospital Shanghai University of Traditional Chinese MedicineLixin Zhu - University at Buffalo, State University of New York
- Resource Type
- Journal article
- Publication Details
- American Journal of Physiology: Cell Physiology, Vol.309(4), pp.C264-C270
- DOI
- 10.1152/ajpcell.00012.2015
- PMID
- 26108660
- ISSN
- 0363-6143
- eISSN
- 1522-1563
- Language
- English
- Date published
- 08/15/2015
- Academic Unit
- Internal Medicine
- Record Identifier
- 9984695681202771
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