Potent induction of rat liver microsomal, drug-metabolizing enzymes by 2,3,3′,4,4′,5-hexabromobiphenyl, a component of fireMaster

L.W Robertson; A Parkinson; S Bandiera; S Safe

doi:10.1016/0009-2797(81)90060-0

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Potent induction of rat liver microsomal, drug-metabolizing enzymes by 2,3,3′,4,4′,5-hexabromobiphenyl, a component of fireMaster

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Potent induction of rat liver microsomal, drug-metabolizing enzymes by 2,3,3′,4,4′,5-hexabromobiphenyl, a component of fireMaster

L.W Robertson, A Parkinson, S Bandiera and S Safe

Chemico-biological interactions, Vol.35(1), pp.13-24

1981

DOI: 10.1016/0009-2797(81)90060-0

PMID: 6258818

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Abstract

The multistep synthesis and purification of 2,3,3′,4,4′,5-hexabromobiphenyl (HBBp) is described. Capillary gas chromatography revealed that HBBp comprises 0.05% of the industrial polybrominated biphenyl (PBB) mixture, fireMaster BP-6 (lot 7062). When administered to immature male Wistar rats, HBBp caused a dose-dependent increase in (a) the activity of benzo[ a]pyrene (B[ a]P) hydroxylase (AHH) and 4-chlorobiphenyl (4-CBP) hydroxylase and (b) the concentration of cytochrome P-450. Sodium dodecyl sulfate (SDS)-gel electrophoresis indicated that these increases in cytochrome P-450 and cytochrome P-450-dependent monooxygenase activities were accompanied by a dose-dependent intensification of a protein of relative molecular weight (Mr) 55 000 which comigrated with the major 3-methylcholanthrene(MC)-inducible form of cytochrome P-450 (i.e., cytochrome P-448). Like MC, but in contrast to phenobarbitone (PB), HBBp competitively displaced 2,3,7,8-[ 3H]tetrachlorodibenzo- p-dioxin ([ 3H]-TCDD) from the cytosolic protein thought to be the receptor for cytochrome P-448 induction. The results indicate that HBBp is a potent inducer of cytochrome P-448 and as such is the third MC-type inducer identified in fireMaster BP-6.

AHH

HEDG

SDS

Master of Science (MS)

TCDD

HBBp

4-CBP

TLC

PCB

PBB

PMR

DMAP

EIC

B[ a]P

Details

Title: Subtitle: Potent induction of rat liver microsomal, drug-metabolizing enzymes by 2,3,3′,4,4′,5-hexabromobiphenyl, a component of fireMaster
Creators: L.W Robertson - School of Public Health, The University of Michigan, Ann Arbor, MI 48109 U.S.A
A Parkinson - The Guelph-Waterloo Centre for Graduate Work in Chemistry, Department of Chemistry, University of Guelph, Guelph, Ontario N1G 2W1 Canada
S Bandiera - The Guelph-Waterloo Centre for Graduate Work in Chemistry, Department of Chemistry, University of Guelph, Guelph, Ontario N1G 2W1 Canada
S Safe - The Guelph-Waterloo Centre for Graduate Work in Chemistry, Department of Chemistry, University of Guelph, Guelph, Ontario N1G 2W1 Canada
Resource Type: Journal article
Publication Details: Chemico-biological interactions, Vol.35(1), pp.13-24
DOI: 10.1016/0009-2797(81)90060-0
PMID: 6258818
NLM abbreviation: Chem Biol Interact
ISSN: 0009-2797
eISSN: 1872-7786
Publisher: Elsevier Ireland Ltd
Language: English
Date published: 1981
Academic Unit: Occupational and Environmental Health
Record Identifier: 9984002441802771

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