Potential mechanism for recruitment and migration of CD133 positive cells to areas of vascular inflammation

Prerna Rastogi; Maureen C White; Alice Rickard; Jane McHowat

doi:10.1016/j.thromres.2008.03.020

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Potential mechanism for recruitment and migration of CD133 positive cells to areas of vascular inflammation

Journal article

Open access

Peer reviewed

Potential mechanism for recruitment and migration of CD133 positive cells to areas of vascular inflammation

Prerna Rastogi, Maureen C White, Alice Rickard and Jane McHowat

Thrombosis research, Vol.123(2), pp.258-266

2008

DOI: 10.1016/j.thromres.2008.03.020

PMCID: PMC2678553

PMID: 18495219

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https://doi.org/10.1016/j.thromres.2008.03.020View

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Abstract

Mast cells are found in large numbers in atherosclerotic plaques. The present study was conducted to determine whether tryptase stimulation of human coronary artery endothelial cells (HCAEC) would lead to an increase in transmigration of CD133 positive cells (CD133+). In vitro these cells can differentiate into mast cells under the influence of specific cytokines and growth factors. CD133+ cells were isolated from umbilical cord blood. They express mRNA for several adhesion molecules that are also utilized in neutrophil migration and can migrate across an HCAEC monolayer. Migration increased significantly when HCAEC were stimulated with tryptase and decreased when CD133+ cells were pretreated with CV3988, a platelet activating factor receptor (PTAFR) antagonist. Following long-term cell culture, these cells stained positively for the presence of tryptase, a mast cell enzyme. CD133+ cells can be utilized as a mast cell precursor population. The transendothelial migration is facilitated by the presence of tryptase and may utilize the PAF/PTAFR interaction in a manner similar to that involved in neutrophil transmigration. Following transmigration, a subset of these progenitor cells may mature into mast cells in the subendothelial space and play a role in propagation of the inflammatory process in atherosclerosis.

Inflammation

AC133 Antigen

Antigens, CD - metabolism

Antigens, CD - ultrastructure

Cell Movement

Cells, Cultured

Coronary Vessels - cytology

Endothelial Cells - cytology

Endothelial Cells - physiology

Endothelium, Vascular - cytology

Glycoproteins - metabolism

Glycoproteins - ultrastructure

Humans

Models, Biological

Peptides - metabolism

RNA, Messenger - metabolism

Vascular Diseases - metabolism

Details

Title: Subtitle: Potential mechanism for recruitment and migration of CD133 positive cells to areas of vascular inflammation
Creators: Prerna Rastogi - Saint Louis University
Maureen C White - Saint Louis University
Alice Rickard - Saint Louis University
Jane McHowat - Saint Louis University
Resource Type: Journal article
Publication Details: Thrombosis research, Vol.123(2), pp.258-266
DOI: 10.1016/j.thromres.2008.03.020
PMID: 18495219
PMCID: PMC2678553
NLM abbreviation: Thromb Res
ISSN: 0049-3848
eISSN: 1879-2472
Grant note: R01 HL068588 / NHLBI NIH HHS HL-68588 / NHLBI NIH HHS R29 HL054907-04 / NHLBI NIH HHS
Language: English
Date published: 2008
Academic Unit: Pathology
Record Identifier: 9984186500502771

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