Potential of berberine to enhance antimicrobial activity of commonly used antibiotics for dairy cow mastitis caused by multiple drug-resistant Staphylococcus epidermidis infection

X Zhou; C Yang; Y Li; X Liu; Y Wang

doi:10.4238/2015.August.19.1

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Potential of berberine to enhance antimicrobial activity of commonly used antibiotics for dairy cow mastitis caused by multiple drug-resistant Staphylococcus epidermidis infection

Journal article

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Peer reviewed

Potential of berberine to enhance antimicrobial activity of commonly used antibiotics for dairy cow mastitis caused by multiple drug-resistant Staphylococcus epidermidis infection

X Zhou, C Yang, Y Li, X Liu and Y Wang

Genetics and molecular research, Vol.14(3), pp.9683-9692

08/19/2015

DOI: 10.4238/2015.August.19.1

PMID: 26345901

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Abstract

Berberine is a plant alkaloid with antimicrobial activity against a variety of microorganisms. In this study, the antimicrobial properties of berberine against multi-drug resistant field isolates of Staphylococcus epidermidis were investigated using berberine alone or in combination with a commonly used antibiotics in veterinary clinics, including penicillin, lincomycin, and amoxicillin. The results indicated that the minimum inhibitory concentrations of berberine, penicillin, lincomycin, and amoxicillin against field S. epidermidis isolates were 2-512, 0.8-213, 0.4-1024, and 0.4-256 mg/mL, respectively. Furthermore, the synergistic effects of antimicrobial activity against these multi-drug resistant isolates were observed when the berberine was combined with penicillin, lincomycin, or amoxicillin; no antagonistic effect of the combination was detected in any of the clinical isolates. These observations were further confirmed using a time-killing assay, in which a combination of 2 agents yielded a greater than 2.03-2.44 log10 decrease in colony-forming unit/mL compared with each agent alone. These findings suggest that berberine is a promising compound for preventing and treating multi-drug resistant S. epidermidis infected mastitis in dairy cows either alone or in combination with other commonly used antibiotics, such as penicillin, lincomycin, and amoxicillin.

Animals

Anti-Bacterial Agents - pharmacology

Berberine - pharmacology

Cattle

Drug Resistance, Multiple, Bacterial

Drug Synergism

Female

Mastitis, Bovine - drug therapy

Mastitis, Bovine - microbiology

Microbial Sensitivity Tests

Staphylococcal Infections - drug therapy

Staphylococcal Infections - microbiology

Staphylococcus epidermidis - drug effects

Time Factors

Details

Title: Subtitle: Potential of berberine to enhance antimicrobial activity of commonly used antibiotics for dairy cow mastitis caused by multiple drug-resistant Staphylococcus epidermidis infection
Creators: X Zhou - Ministry of Education of the People's Republic of China
C Yang - Ministry of Education of the People's Republic of China
Y Li - Ministry of Education of the People's Republic of China
X Liu - Ministry of Education of the People's Republic of China
Y Wang - Ministry of Education of the People's Republic of China
Resource Type: Journal article
Publication Details: Genetics and molecular research, Vol.14(3), pp.9683-9692
DOI: 10.4238/2015.August.19.1
PMID: 26345901
NLM abbreviation: Genet Mol Res
ISSN: 1676-5680
eISSN: 1676-5680
Language: English
Date published: 08/19/2015
Academic Unit: Anatomy and Cell Biology
Record Identifier: 9984284330602771

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