Practical reconstruction method for bioluminescence tomography

Wenxiang Cong; Ge Wang; Durairaj Kumar; Yi Liu; Ming Jiang; Lihong Wang; Eric Hoffman; Geoffrey McLennan; Paul McCray; Joseph Zabner; Alexander Cong

doi:10.1364/OPEX.13.006756

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Practical reconstruction method for bioluminescence tomography

Journal article

Open access

Peer reviewed

Practical reconstruction method for bioluminescence tomography

Wenxiang Cong, Ge Wang, Durairaj Kumar, Yi Liu, Ming Jiang, Lihong Wang, Eric Hoffman, Geoffrey McLennan, Paul McCray, Joseph Zabner, …

Optics express, Vol.13(18), pp.6756-6771

09/05/2005

DOI: 10.1364/OPEX.13.006756

PMID: 19498692

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Abstract

Bioluminescence tomography (BLT) is used to localize and quantify bioluminescent sources in a small living animal. By advancing bioluminescent imaging to a tomographic framework, it helps to diagnose diseases, monitor therapies and facilitate drug development. In this paper, we establish a direct linear relationship between measured surface photon density and an unknown bioluminescence source distribution by using a finite-element method based on the diffusion approximation to the photon propagation in biological tissue. We develop a novel reconstruction algorithm to recover the source distribution. This algorithm incorporates a priori knowledge to define the permissible source region in order to enhance numerical stability and efficiency. Simulations with a numerical mouse chest phantom demonstrate the feasibility of the proposed BLT algorithm and reveal its performance in terms of source location, density, and robustness against noise. Lastly, BLT experiments are performed to identify the location and power of two light sources in a physical mouse chest phantom.

Details

Title: Subtitle: Practical reconstruction method for bioluminescence tomography
Creators: Wenxiang Cong
Ge Wang
Durairaj Kumar
Yi Liu
Ming Jiang
Lihong Wang
Eric Hoffman
Geoffrey McLennan
Paul McCray
Joseph Zabner
Alexander Cong
Resource Type: Journal article
Publication Details: Optics express, Vol.13(18), pp.6756-6771
DOI: 10.1364/OPEX.13.006756
PMID: 19498692
ISSN: 1094-4087
eISSN: 1094-4087
Grant note: R21 EB001685 / NIBIB NIH HHS
Language: English
Date published: 09/05/2005
Academic Unit: Roy J. Carver Department of Biomedical Engineering; Radiology; Pulmonary, Critical Care, and Occupational Medicine; Microbiology and Immunology; Pulmonary Medicine; Stead Family Department of Pediatrics; Internal Medicine
Record Identifier: 9984093455402771

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