Journal article
Precipitant-Controlled Growth of Lysozyme Crystals in Sodium Thiocyanate
Crystal growth & design, Vol.4(6), pp.1387-1393
11/01/2004
DOI: 10.1021/cg0341297
Abstract
We have shown previously that large high-quality single protein crystals can be grown by varying temperature in a predetermined fashion such that the solution supersaturation and the rate of crystal growth are maintained at a constant value in batch crystallization systems. Here we have adapted the constant supersaturation control (CSC) methodology to isothermal crystal growth. In this version of the CSC protocol, dynamic control is provided by changing precipitant concentration through a dialysis membrane. We have developed a system of multiple growth cells in series that allows a variety of precipitant concentration trajectories to be sampled simultaneously during a single CSC experiment. Several different concentration profiles were used to grow lysozyme crystals in NaSCN salt. Increasing the precipitant concentration using a slow linear profile results in the highest quality crystals while requiring the least amount of characterization work. Coupling this type of profile with multiple growth cells in series appears to be a promising method for dynamically controlling protein crystal growth isothermally.
Details
- Title: Subtitle
- Precipitant-Controlled Growth of Lysozyme Crystals in Sodium Thiocyanate
- Creators
- Wayne F Jones - Chemical and Biochemical Engineering, University of Iowa, 4122 Seamans Center, Iowa City, Iowa 52242Mark A Arnold - Chemical and Biochemical Engineering, University of Iowa, 4122 Seamans Center, Iowa City, Iowa 52242John M Wiencek - Chemical and Biochemical Engineering, University of Iowa, 4122 Seamans Center, Iowa City, Iowa 52242
- Resource Type
- Journal article
- Publication Details
- Crystal growth & design, Vol.4(6), pp.1387-1393
- DOI
- 10.1021/cg0341297
- ISSN
- 1528-7483
- eISSN
- 1528-7505
- Language
- English
- Date published
- 11/01/2004
- Academic Unit
- Fraternal Order of Eagles Diabetes Research Center; Chemistry; Center for Biocatalysis and Bioprocessing
- Record Identifier
- 9984216672202771
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