Precise prediction of a dominant class I MHC-restricted epitope of Listeria monocytogenes

Eric G Pamer; John T Harty; Michael J Bevan

doi:10.1038/353852a0

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Precise prediction of a dominant class I MHC-restricted epitope of Listeria monocytogenes

Journal article

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Precise prediction of a dominant class I MHC-restricted epitope of Listeria monocytogenes

Eric G Pamer, John T Harty and Michael J Bevan

Nature (London), Vol.353(6347), pp.852-855

10/31/1991

DOI: 10.1038/353852a0

PMCID: PMC2782917

PMID: 1719425

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Abstract

Listeria monocytogenes is a Gram-positive bacterium which grows in the cytoplasm of eukaryotic cells and can cause severe disease in immunocompromised individuals 1 , 2 . In murine systems CD8 + T lymphocytes have been shown to be important effectors of acquired protective immunity against L. monocytogenes 3 – 5 . Class I MHC-restricted CD8 + cytotoxic T lymphocytes (CTL), which lyse J774 macrophage-like targets infected with L. monocytogenes , are induced following in vivo injection of live organisms. Natural peptide epitopes derived from L. monocytogenes can be acid-extracted from heavily infected BALB/c spleens and detected by CTL. A CTL clone, B9, derived from a (BALB/c × C57BL/6)F 1 , (H–2 d×b ) mouse, recognizes one of these natural epitopes in an H–2K d -restricted fashion. B9 also recognizes P815 (H–2 d ) mastocytoma cells transfected with the listeriolysin gene. To identify the region of the listeriolysin recognized by CTL we used the H–2K d peptide-binding motif described by Rammensee and colleagues 6 to synthesize 11 nonamer peptides. One of these peptides, listeriolysin 91–99, was recognized very efficiently by B9. This represents the first identified class I MHC-restricted epitope of bacteria and demonstrates the utility of the allele-specific motif for predicting CTL epitopes.

Details

Title: Subtitle: Precise prediction of a dominant class I MHC-restricted epitope of Listeria monocytogenes
Creators: Eric G Pamer - Howard Hughes Medical Institute, Department of Immunology, University of Washington, Seattle, Washington 98195, USA
John T Harty - Howard Hughes Medical Institute, Department of Immunology, University of Washington, Seattle, Washington 98195, USA
Michael J Bevan - Howard Hughes Medical Institute, Department of Immunology, University of Washington, Seattle, Washington 98195, USA
Resource Type: Journal article
Publication Details: Nature (London), Vol.353(6347), pp.852-855
DOI: 10.1038/353852a0
PMID: 1719425
PMCID: PMC2782917
NLM abbreviation: Nature
ISSN: 0028-0836
eISSN: 1476-4687
Language: English
Date published: 10/31/1991
Academic Unit: Pathology
Record Identifier: 9984047773902771

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