Predicting CD62L expression during the CD8+ T cell response in vivo

Timothy E Schlub; Vladimir P Badovinac; Jaime T Sabel; John T Harty; Miles P Davenport

doi:10.1038/icb.2009.80

Back

Predicting CD62L expression during the CD8+ T cell response in vivo

Journal article

Open access

Peer reviewed

Predicting CD62L expression during the CD8+ T cell response in vivo

Timothy E Schlub, Vladimir P Badovinac, Jaime T Sabel, John T Harty and Miles P Davenport

Immunology and cell biology, Vol.88(2), pp.157-164

02/2010

DOI: 10.1038/icb.2009.80

PMCID: PMC2824781

PMID: 19859082

Files and links (1)

url

http://doi.org/10.1038/icb.2009.80View

Open Access

Abstract

Acute infection leads to CD8+ T cell activation, division, and differentiation. Following clearance of infection, cells revert to two distinct subsets of memory, central (T CM ) and effector (T EM ) memory. Adoptive transfer of naïve T cell receptor transgenic (TCR-tg) T cells has been used to study the differentiation of these memory subsets, which are often discriminated by expression of CD62L. Naïve CD8+ T cells are CD62L high , and CD62L expression is lost during the ‘effector’ phase. Adoptive transfer studies show that higher transfer frequencies result in diminished T cell expansion and a higher proportion CD62L high . This suggests a relationship between CD62L expression and cell division, where division leads to conversion from CD62L high to CD62L low phenotype. To address this hypothesis we adoptively transferred graded numbers of OT-1 TCR-tg T cells from naïve donors and tracked the kinetics and phenotype of the immune response following infection. We developed a simple mathematical model of division-linked CD62L differentiation which we compared to the experimental data. Our results show that division-linked differentiation predicts the differences in proportion of cells CD62L high observed between responses of different adoptive transfer number, and within individual mice. We calculate that approximately 20% of CD62L high cells convert to CD62L low during each division.

Cell Differentiation

Cellular proliferation

Mathematical model

CD8 T cells

Details

Title: Subtitle: Predicting CD62L expression during the CD8+ T cell response in vivo
Creators: Timothy E Schlub - Complex Systems in Biology Group, Centre for Vascular Research, University of New South Wales, Kensington NSW 2052, Australia
Vladimir P Badovinac - Department of Pathology, University of Iowa, Iowa city, IA 52242, USA
Jaime T Sabel - Department of Pathology, University of Iowa, Iowa city, IA 52242, USA
John T Harty - Department of Microbiology, University of Iowa, Iowa city, IA 52242, USA
Miles P Davenport - Complex Systems in Biology Group, Centre for Vascular Research, University of New South Wales, Kensington NSW 2052, Australia
Resource Type: Journal article
Publication Details: Immunology and cell biology, Vol.88(2), pp.157-164
DOI: 10.1038/icb.2009.80
PMID: 19859082
PMCID: PMC2824781
NLM abbreviation: Immunol Cell Biol
ISSN: 0818-9641
eISSN: 1440-1711
Language: English
Date published: 02/2010
Academic Unit: Microbiology and Immunology; Pathology
Record Identifier: 9984047874102771

Metrics

17 Record Views

20 Times Cited - Web of Science