Journal article
Predicting oligonucleotide affinity to nucleic acid targets
RNA (Cambridge), Vol.5(11), pp.1458-1469
11/1999
DOI: 10.1017/S1355838299991148
PMCID: PMC1369867
PMID: 10580474
Abstract
A computer program, OligoWalk, is reported that
predicts the equilibrium affinity of complementary DNA
or RNA oligonucleotides to an RNA target. This program
considers the predicted stability of the oligonucleotide-target
helix and the competition with predicted secondary structure
of both the target and the oligonucleotide. Both unimolecular
and bimolecular oligonucleotide self structure are considered
with a user-defined concentration. The application of OligoWalk
is illustrated with three comparisons to experimental results
drawn from the literature.
Details
- Title: Subtitle
- Predicting oligonucleotide affinity to nucleic acid targets
- Creators
- DAVID H. Mathews - University of RochesterMARK E. Burkard - University of RochesterSUSAN M. Freier - Division of Molecular and Structural Biology, Isis Pharmaceuticals, Carlsbad, California 92008, USAJACQUELINE R. Wyatt - Division of Molecular and Structural Biology, Isis Pharmaceuticals, Carlsbad, California 92008, USADOUGLAS H. Turner - University of Rochester
- Resource Type
- Journal article
- Publication Details
- RNA (Cambridge), Vol.5(11), pp.1458-1469
- DOI
- 10.1017/S1355838299991148
- PMID
- 10580474
- PMCID
- PMC1369867
- NLM abbreviation
- RNA
- ISSN
- 1355-8382
- eISSN
- 1469-9001
- Publisher
- Cambridge University Press
- Number of pages
- 12
- Language
- English
- Date published
- 11/1999
- Academic Unit
- Internal Medicine
- Record Identifier
- 9984701255202771
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