Journal article
Preferential Proliferation and Differentiation of Double-Positive Thymocytes into CD8+ Single-Positive Thymocytes in a Novel Cell Culture Medium
Cellular immunology, Vol.202(1), pp.41-53
05/25/2000
DOI: 10.1006/cimm.2000.1656
PMID: 10873305
Abstract
The identification of factors that regulate the proliferation and differentiation of double-positive (DP) into CD4+ and CD8+ single-positive (SP) thymocytes has proven difficult due to the inability of DP thymocytes to proliferate, expand, and differentiate into SP thymocytes in available cell culture media. Here we report on the ability of DP thymocytes to differentiate in a novel conditioned medium, termed XLCM, derived from the supernatant of mitogen activated human cord blood mononuclear cells. During a 5-day culture in XLCM in the absence of thymic stromal cells, DP thymocytes from normal mice and MHC double knockout mice (lack SP thymocytes) proliferate, expand, and differentiate into several (αβTCR+, NK1.1+αβTCR+, and γδTCR+) subsets of CD4+ and predominantly CD8+ SP thymocytes. These studies suggest that the use of XLCM may aid in the characterization of factors that regulate the differentiation of DP thymocytes into CD8+ SP thymocytes.
Details
- Title: Subtitle
- Preferential Proliferation and Differentiation of Double-Positive Thymocytes into CD8+ Single-Positive Thymocytes in a Novel Cell Culture Medium
- Creators
- Jian-Xin Gao - Autoimmunity/Diabetes Group, The John P. Robarts Research Institute, The University of Western Ontario, London, Ontario, N6G 2V4, CanadaJian Zhang - Autoimmunity/Diabetes Group, The John P. Robarts Research Institute, The University of Western Ontario, London, Ontario, N6G 2V4, CanadaChristian Awaraji - Gene Therapy and Molecular Virology Group, The John P. Robarts Research Institute, The University of Western Ontario, London, Ontario, N6G 2V4, CanadaMickie Bhatia - Gene Therapy and Molecular Virology Group, The John P. Robarts Research Institute, The University of Western Ontario, London, Ontario, N6G 2V4, CanadaAnthony Jevnikar - Transplantation Biology Group, The John P. Robarts Research Institute, The University of Western Ontario, London, Ontario, N6G 2V4, CanadaBhagirath Singh - The John P. Robarts Research Institute, Department of Microbiology and Immunology and Medicine, The University of Western Ontario, London, Ontario, N6G 2V4, CanadaDavid Bell - Hemosol Inc. Etobicoke, Ontario, M9W 4Z7, CanadaTerry L Delovitch - Autoimmunity/Diabetes Group, The John P. Robarts Research Institute, The University of Western Ontario, London, Ontario, N6G 2V4, Canada
- Resource Type
- Journal article
- Publication Details
- Cellular immunology, Vol.202(1), pp.41-53
- Publisher
- Elsevier Inc
- DOI
- 10.1006/cimm.2000.1656
- PMID
- 10873305
- ISSN
- 0008-8749
- eISSN
- 1090-2163
- Language
- English
- Date published
- 05/25/2000
- Academic Unit
- Pathology
- Record Identifier
- 9984046906902771
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