Preferential infection of mature dendritic cells by the JHM strain of mouse hepatitis virus

Haixia Zhou; Stanley Perlman

doi:10.1007/978-0-387-33012-9_74

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Preferential infection of mature dendritic cells by the JHM strain of mouse hepatitis virus

Journal article

Open access

Peer reviewed

Preferential infection of mature dendritic cells by the JHM strain of mouse hepatitis virus

Haixia Zhou and Stanley Perlman

Advances in experimental medicine and biology, Vol.581, pp.411-414

2006

DOI: 10.1007/978-0-387-33012-9_74

PMID: 17037570

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https://doi.org/10.1007/978-0-387-33012-9_74View

Published (Version of record) Open Access

Abstract

Mouse hepatitis virus strain JHM (MHV-JHM) causes acute encephalitis and acute and chronic demyelinating diseases in mice. Dendritic cells (DCs) are key cells in the initiation of innate and adaptive immune responses, and infection of these cells could potentially contribute to a dysregulated immune response; consistent with this, recent results suggest that DCs are readily infected by another strain of mouse hepatitis virus, the A59 strain (MHV-A59). Herein, we show that the JHM strain also productively infected DCs. Moreover, mature DCs were at least 10 times more susceptible than immature DCs to infection with MHV-JHM. DC function was impaired after MHV-JHM infection, resulting in decreased stimulation of CD8 T cells in vitro. Preferential infection of mature DCs was not due to differential expression of the MHV-JHM receptor CEACAM-1a on mature or immature cells or to differences in apoptosis. Although we could not detect infected DCs in vivo, both CD8(+) and CD11b(+) splenic DCs were susceptible to infection with MHV-JHM directly ex vivo. This preferential infection of mature DCs may inhibit the development of an efficient immune response to the virus.

Flow Cytometry

Cell Line

Green Fluorescent Proteins - metabolism

Murine hepatitis virus - metabolism

Murine hepatitis virus - pathogenicity

Cell Adhesion Molecules - metabolism

Antigens, CD - metabolism

Animals

Coronavirus Infections - blood

Mice

Dendritic Cells - virology

CD11c Antigen - physiology

T-Lymphocytes, Regulatory - virology

Details

Title: Subtitle: Preferential infection of mature dendritic cells by the JHM strain of mouse hepatitis virus
Creators: Haixia Zhou - University of Iowa, Iowa City 52242, USA
Stanley Perlman
Resource Type: Journal article
Publication Details: Advances in experimental medicine and biology, Vol.581, pp.411-414
DOI: 10.1007/978-0-387-33012-9_74
PMID: 17037570
NLM abbreviation: Adv Exp Med Biol
eISBN: 0387330127; 9780387330129
ISSN: 0065-2598
Publisher: United States
Language: English
Date published: 2006
Academic Unit: Microbiology and Immunology; Stead Family Department of Pediatrics; Iowa Neuroscience Institute; Infectious Disease (Pediatrics)
Record Identifier: 9983777473302771

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