Preferential infection of mature dendritic cells by mouse hepatitis virus strain JHM

Haixia Zhou; Stanley Perlman

doi:10.1128/JVI.80.5.2506-2514.2006

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Preferential infection of mature dendritic cells by mouse hepatitis virus strain JHM

Journal article

Open access

Peer reviewed

Preferential infection of mature dendritic cells by mouse hepatitis virus strain JHM

Haixia Zhou and Stanley Perlman

Journal of virology, Vol.80(5), pp.2506-2514

03/2006

DOI: 10.1128/JVI.80.5.2506-2514.2006

PMCID: PMC1395395

PMID: 16474157

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Abstract

Mouse hepatitis virus strain JHM (MHV-JHM) causes acute encephalitis and acute and chronic demyelinating diseases in mice. Dendritic cells (DCs) are key cells in the initiation of innate and adaptive immune responses, and infection of these cells could potentially contribute to a dysregulated immune response; consistent with this, recent results suggest that DCs are readily infected by another strain of mouse hepatitis virus, the A59 strain (MHV-A59). Herein, we show that the JHM strain also productively infected DCs. Moreover, mature DCs were at least 10 times more susceptible than immature DCs to infection with MHV-JHM. DC function was impaired after MHV-JHM infection, resulting in decreased stimulation of CD8 T cells in vitro. Preferential infection of mature DCs was not due to differential expression of the MHV-JHM receptor CEACAM-1a on mature or immature cells or to differences in apoptosis. Although we could not detect infected DCs in vivo, both CD8(+) and CD11b(+) splenic DCs were susceptible to infection with MHV-JHM directly ex vivo. This preferential infection of mature DCs may inhibit the development of an efficient immune response to the virus.

Green Fluorescent Proteins - analysis

Dendritic Cells - immunology

Mice, Inbred C57BL

Green Fluorescent Proteins - genetics

Murine hepatitis virus - growth & development

Receptors, Virus - analysis

Carcinoembryonic Antigen - analysis

Coronavirus Infections - immunology

Animals

Flow Cytometry

Coronavirus Infections - virology

Coronavirus Infections - pathology

Mice

CD8-Positive T-Lymphocytes - immunology

Dendritic Cells - virology

Genes, Reporter

Apoptosis

Disease Models, Animal

Details

Title: Subtitle: Preferential infection of mature dendritic cells by mouse hepatitis virus strain JHM
Creators: Haixia Zhou - Department of Pediatrics, University of Iowa, Iowa City, 52242, USA
Stanley Perlman
Resource Type: Journal article
Publication Details: Journal of virology, Vol.80(5), pp.2506-2514
DOI: 10.1128/JVI.80.5.2506-2514.2006
PMID: 16474157
PMCID: PMC1395395
NLM abbreviation: J Virol
ISSN: 0022-538X
eISSN: 1098-5514
Publisher: United States
Grant note: R01 NS36592 / NINDS NIH HHS R01 NS036592 / NINDS NIH HHS
Language: English
Date published: 03/2006
Academic Unit: Microbiology and Immunology; Stead Family Department of Pediatrics; Iowa Neuroscience Institute; Infectious Disease (Pediatrics)
Record Identifier: 9983777473402771

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