Journal article
Preparation and evaluation of human choroid extracellular matrix scaffolds for the study of cell replacement strategies
Acta Biomaterialia, Vol.57, pp.293-303
07/15/2017
DOI: 10.1016/j.actbio.2017.05.011
PMCID: PMC5515248
PMID: 28483697
Abstract
[Display omitted] Endothelial cells (ECs) of the choriocapillaris are one of the first cell types lost during age-related macular degeneration (AMD), and cell replacement therapy is currently a very promising option for patients with advanced AMD. We sought to develop a reliable method for the production of human choroidal extracellular matrix (ECM) scaffolds, which will allow for the study of choroidal EC (CEC) replacement strategies in an environment that closely resembles the native tissue. Human RPE/choroid tissue was treated sequentially with Triton X-100, SDS, and DNase to remove all native cells. While all cells were successfully removed from the tissue, collagen IV, elastin, and laminin remained, with preserved architecture of the acellular vascular tubes. The ECM scaffolds were then co-cultured with exogenous ECs to determine if the tissue can support cell growth and allow EC reintegration into the decellularized choroidal vasculature. Both monkey and human ECs took up residence in the choriocapillary tubes of the decellularized tissue. Together, these data suggest that our decellularization methods are sufficient to remove all cellular material yet gentle enough to preserve tissue structure and allow for the optimization of cell replacement strategies. Age-related macular degeneration (AMD) is a devastating disease affecting more than 600 million people worldwide. Endothelial cells of the choriocapillaris (CECs) are among the first cell types lost in early AMD, and cell replacement therapy is currently the most promising option for restoring vision in patients with advanced AMD. In order to study CEC replacement strategies we have generated a 3D choroid scaffold using a novel decellularization method in human RPE/choroid tissue. To our knowledge, this is the first report describing decellularization of human RPE/choroid, as well as recellularization of a choroid scaffold with CECs. This work will aid in our development and optimization of cell replacement strategies using a tissue scaffold that is similar to the in vivo environment.
Details
- Title: Subtitle
- Preparation and evaluation of human choroid extracellular matrix scaffolds for the study of cell replacement strategies
- Creators
- Kathleen R Chirco - University of IowaKristan S Worthington - University of Iowa, The University of Iowa Institute for Vision ResearchMiles J Flamme-Wiese - University of Iowa, The University of Iowa Institute for Vision ResearchMegan J Riker - University of Iowa, The University of Iowa Institute for Vision ResearchJoshua D Andrade - New York UniversityBeatrix M Ueberheide - New York UniversityEdwin M Stone - University of Iowa, The University of Iowa Institute for Vision ResearchBudd A Tucker - University of Iowa, The University of Iowa Institute for Vision ResearchRobert F Mullins - University of Iowa, The University of Iowa Institute for Vision Research
- Resource Type
- Journal article
- Publication Details
- Acta Biomaterialia, Vol.57, pp.293-303
- DOI
- 10.1016/j.actbio.2017.05.011
- PMID
- 28483697
- PMCID
- PMC5515248
- NLM abbreviation
- Acta Biomater
- ISSN
- 1742-7061
- eISSN
- 1878-7568
- Publisher
- Elsevier Ltd
- Grant note
- DOI: 10.13039/100000002, name: NIH, award: EY024605; name: Elmer and Sylvia Sramek Charitable Foundation
- Language
- English
- Date published
- 07/15/2017
- Academic Unit
- Roy J. Carver Department of Biomedical Engineering; Iowa Neuroscience Institute; Chemical and Biochemical Engineering; Ophthalmology and Visual Sciences
- Record Identifier
- 9983979950102771
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