Journal article
Preparation and in vivo characterization of (MnCl2)-Mn-51 as PET tracer of Ca2+ channel-mediated transport
Scientific reports, Vol.7(1), 3033
06/08/2017
DOI: 10.1038/s41598-017-03202-0
PMCID: PMC5465055
PMID: 28596540
Abstract
Manganese has long been employed as a T-1-shortening agent in magnetic resonance imaging (MRI) applications, but these techniques are limited by the biotoxicity of bulk-manganese. Positron emission tomography (PET) offers superior contrast sensitivity compared with MRI, and recent preclinical PET studies employing Mn-52g (t(1/2): 5.6 d, beta(+): 29%) show promise for a variety of applications including cell tracking, neural tract tracing, immunoPET, and functional beta-cell mass quantification. The half-life and confounding gamma emissions of Mn-52g are prohibitive to clinical translation, but the short-lived Mn-51 (t(1/2): 46 min, beta(+): 97%) represents a viable alternative. This work develops methods to produce Mn-51 on low-energy medical cyclotrons, characterizes the in vivo behavior of (MnCl2)-Mn-51 in mice, and performs preliminary human dosimetry predictions. Mn-51 was produced by proton irradiation of electrodeposited isotopically-enriched Fe-54 targets. Radiochemically isolated (MnCl2)-Mn-51 was intravenously administered to ICR mice which were scanned by dynamic and static PET, followed by ex vivo gamma counting. Rapid blood clearance was observed with stable uptake in the pancreas, kidneys, liver, heart, and salivary gland. Dosimetry calculations predict that 370 MBq of Mn-51 in an adult human male would yield an effective dose equivalent of approximately 13.5 mSv, roughly equivalent to a clinical [F-18]-FDG procedure.
Details
- Title: Subtitle
- Preparation and in vivo characterization of (MnCl2)-Mn-51 as PET tracer of Ca2+ channel-mediated transport
- Creators
- Stephen A. Graves - Univ Wisconsin, Dept Med Phys, 1111 Highland Ave, Madison, WI 53705 USAReinier Hernandez - University of Wisconsin–MadisonHector F. Valdovinos - University of Wisconsin–MadisonPaul A. Ellison - University of Wisconsin–MadisonJonathan W. Engle - University of Wisconsin–MadisonTodd E. Barnhart - University of Wisconsin–MadisonWeibo Cai - University of Wisconsin–MadisonRobert J. Nickles - University of Wisconsin–Madison
- Resource Type
- Journal article
- Publication Details
- Scientific reports, Vol.7(1), 3033
- DOI
- 10.1038/s41598-017-03202-0
- PMID
- 28596540
- PMCID
- PMC5465055
- NLM abbreviation
- Sci Rep
- ISSN
- 2045-2322
- eISSN
- 2045-2322
- Publisher
- Springer Nature
- Number of pages
- 8
- Grant note
- T32GM008349 / NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Institute of General Medical Sciences (NIGMS) DGE-1256259 / National Science Foundation; National Science Foundation (NSF) P30CA014520 / NATIONAL CANCER INSTITUTE; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Cancer Institute (NCI) University of Wisconsin - Madison T32-CA009206; R01-CA169365; P30-CA014520; T32-GM008349 / National Institutes of Health; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA 125246-RSG-13-099-01-CCE / American Cancer Society
- Language
- English
- Date published
- 06/08/2017
- Academic Unit
- Roy J. Carver Department of Biomedical Engineering; Radiology; Radiation Oncology
- Record Identifier
- 9984383290602771
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