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Preparative purification of a high-mannose type N-glycan from soy bean agglutinin by hydrazinolysis and tyrosinamide derivatization
Journal article   Peer reviewed

Preparative purification of a high-mannose type N-glycan from soy bean agglutinin by hydrazinolysis and tyrosinamide derivatization

David L Evers, Rex L Hung, V Hayden Thomas and Kevin G Rice
Analytical biochemistry, Vol.265(2), pp.313-316
12/15/1998
DOI: 10.1006/abio.1998.2895
PMID: 9882408

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Abstract

The N-linked oligosaccharide from soy bean agglutinin (Man9) was isolated on a preparative scale following derivatization with Boc-tyrosine. The procedure utilized preparative hydrazinolysis to release the oligosaccharide and yielded multi-micromol quantities of Boc-tyrosine-Man9 which was characterized by 1H NMR and ES-MS.
Carbohydrate Sequence Magnetic Resonance Spectroscopy Soybean Proteins Molecular Sequence Data Chromatography, High Pressure Liquid Tyrosine - analogs & derivatives Hydrazines - chemistry Plant Lectins Polysaccharides - isolation & purification Polysaccharides - chemistry Lectins - chemistry Mass Spectrometry - methods Tyrosine - chemistry Mannose - chemistry

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