Journal article
Presence of Clostridioides difficile and multidrug-resistant healthcare-associated pathogens in stool specimens from hospitalized patients in the USA
The Journal of hospital infection, Vol.106(1), pp.179-185
09/2020
DOI: 10.1016/j.jhin.2020.07.003
PMID: 32649974
Abstract
Healthcare-associated infections (HCAIs) continue to be a major cause of morbidity and mortality. Many HCAI pathogens, including multidrug-resistant organisms (MDROs), colonize the gastrointestinal tract.
To determine the frequency of MDRO carriage in patients who do and do not harbour toxigenic Clostridioides difficile in their stools.
Stool specimens received from nine US laboratories were cultured using media selective for C. difficile, Staphylococcus aureus, vancomycin-resistant enterococci (VRE), and carbapenem-resistant Gram-negative organisms (CROs). Specimens and isolates were also tested by polymerase chain reaction (PCR). Bacterial isolates underwent susceptibility testing and genotyping.
Among 363 specimens, 175 yielded toxigenic C. difficile isolates spanning 27 PCR ribotypes. C. difficile (TCD+) stools harboured an additional 28 organisms, including six CROs (3.4%), of which two (1.1%) were carbapenemase-producing organisms (CPOs), 19 VRE (10.9%), and three meticillin-resistant S. aureus isolates (MRSA, 1.7 %). Stools that were culture negative for toxigenic C. difficile (TCD–) yielded 26 organisms, including four CROs (2.1%), 20 VRE (10.6), and two MRSA (1.1%). Excluding C. difficile, no significant differences were seen in the rates of the MDROs between TCD+ and TCD– specimens.
Overall, 15.4% of the TCD+ stools and 11.2% of the TCD– stools carried at least one non-C. difficile MDRO pathogen, indicating that multiple MDROs may be present in the gastrointestinal tracts of patients, including those that harbour C. difficile.
Details
- Title: Subtitle
- Presence of Clostridioides difficile and multidrug-resistant healthcare-associated pathogens in stool specimens from hospitalized patients in the USA
- Creators
- I.A Tickler - CEPHEIDC.M dela Cruz - Cepheid, Sunnyvale, CA, USAA.E Obradovich - Creighton UniversityR.V Goering - Creighton UniversityS Dewell - CEPHEIDV.M Le - CEPHEIDF.C Tenover - CEPHEIDJ AndersonN BanaeiM BankowskiJ.L DillA.T HarringtonM.A HenthorneJ.S KluttsT KoyamatsuR OvercastV.J RekasiusA RojtmanHealthcare Associated Infections Consortium
- Resource Type
- Journal article
- Publication Details
- The Journal of hospital infection, Vol.106(1), pp.179-185
- Publisher
- Elsevier Ltd
- DOI
- 10.1016/j.jhin.2020.07.003
- PMID
- 32649974
- ISSN
- 0195-6701
- eISSN
- 1532-2939
- Grant note
- name: Cepheid; name: Cepheid
- Language
- English
- Date published
- 09/2020
- Academic Unit
- Pathology
- Record Identifier
- 9984186282202771
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