Prevalence and genetic relatedness of methicillin-susceptible Staphylococcus aureus isolates detected by the Xpert MRSA nasal assay

Sophie S Arbefeville; Kunyan Zhang; Jennifer S Kroeger; Wanita J Howard; Daniel J Diekema; Sandra S Richter

doi:10.1128/JCM.00046-11

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Prevalence and genetic relatedness of methicillin-susceptible Staphylococcus aureus isolates detected by the Xpert MRSA nasal assay

Journal article

Open access

Peer reviewed

Prevalence and genetic relatedness of methicillin-susceptible Staphylococcus aureus isolates detected by the Xpert MRSA nasal assay

Sophie S Arbefeville, Kunyan Zhang, Jennifer S Kroeger, Wanita J Howard, Daniel J Diekema and Sandra S Richter

Journal of clinical microbiology, Vol.49(8), pp.2996-2999

08/2011

DOI: 10.1128/JCM.00046-11

PMCID: PMC3147776

PMID: 21677066

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Abstract

Methicillin-susceptible Staphylococcus aureus (MSSA) isolates lacking mecA yet testing positive on the Xpert MRSA assay were recovered from culture for 7.7% of 248 Xpert-positive nasal samples. These "false-positive" Xpert results may be attributed to staphylococcal cassette chromosome (SCC) elements without the mecA gene. Pulsed-field gel electrophoresis (PFGE) analysis revealed a diverse population of MSSA strains.

Penicillin-Binding Proteins

Molecular Diagnostic Techniques - methods

DNA, Bacterial - genetics

Staphylococcus aureus - isolation & purification

Bacteriological Techniques - methods

Diagnostic Errors

Humans

Bacterial Proteins - genetics

Anti-Bacterial Agents - pharmacology

Staphylococcal Infections - microbiology

Methicillin - pharmacology

Staphylococcus aureus - drug effects

Details

Title: Subtitle: Prevalence and genetic relatedness of methicillin-susceptible Staphylococcus aureus isolates detected by the Xpert MRSA nasal assay
Creators: Sophie S Arbefeville - Department of Clinical Pathology, Cleveland Clinic, 9500 Euclid Avenue, L40, Cleveland, OH 44195, USA
Kunyan Zhang
Jennifer S Kroeger
Wanita J Howard
Daniel J Diekema
Sandra S Richter
Resource Type: Journal article
Publication Details: Journal of clinical microbiology, Vol.49(8), pp.2996-2999
DOI: 10.1128/JCM.00046-11
PMID: 21677066
PMCID: PMC3147776
NLM abbreviation: J Clin Microbiol
ISSN: 1098-660X
eISSN: 1098-660X
Publisher: United States
Language: English
Date published: 08/2011
Academic Unit: Infectious Diseases; Pathology; Internal Medicine
Record Identifier: 9983986271302771

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