Journal article
Prevalence and molecular characterization of Staphylococcus aureus in commercially available meat over a one-year period in Iowa, USA
Food microbiology, Vol.65, pp.122-129
08/2017
DOI: 10.1016/j.fm.2017.01.015
PMID: 28399994
Abstract
Methicillin-resistant Staphylococcus aureus (MRSA) is a leading cause of infectious disease morbidity and mortality. Previous studies have confirmed the presence of S. aureus, including MRSA, on raw meat products. We investigated the prevalence and molecular epidemiology of S. aureus and MRSA in commercially-distributed antibiotic-free and conventional raw meat products (n = 3290) purchased in 8 Iowa retail stores weekly for a period of one year. Isolates were characterized using spa typing, and PCR was used to detect the presence of the Panton-Valentine leukocidin (PVL) and mecA genes. Quantitation of S. aureus on meat products was carried out one week per month. The prevalence of S. aureus on meat samples was 27.8% (913/3290). Compared to antibiotic-free meat samples, higher prevalence of both MRSA and methicillin-susceptible S. aureus (MSSA) were found in conventional meat samples. Among the S. aureus isolates, 18 were PVL-positive (1.9%) and 41 (4.5%) carried mecA. Phenotypic oxacillin resistance was observed for 17.1% (41/239) of the isolates tested, while 23% (55/239) were multi-drug resistant. A total of 132 spa types were detected from 913 contaminated meat samples. Overall, t002 was the most common spa type identified (137; 15.0%). The number of colony-forming units (CFU) per 10 g meat ranged from 2 to 517 (median: 8 CFU per 10 g of meat; mean: 28) with the highest bacterial load observed on turkey samples. These data reinforce the need to consider meat products as potential vehicles of S. aureus transmission from farm into human households, and the potential need for public health intervention programs pre and post-slaughter in meat processing facilities.
Details
- Title: Subtitle
- Prevalence and molecular characterization of Staphylococcus aureus in commercially available meat over a one-year period in Iowa, USA
- Creators
- Dipendra Thapaliya - Department of Epidemiology, University of Iowa College of Public Health, 145N. Riverside Drive, Iowa City, IA, 52242, United States; Center for Emerging Infectious Diseases, University of Iowa College of Public Health, 2501 Crosspark Rd, Coralville, IA, 52241, United StatesBrett M Forshey - Department of Epidemiology, University of Iowa College of Public Health, 145N. Riverside Drive, Iowa City, IA, 52242, United States; Center for Emerging Infectious Diseases, University of Iowa College of Public Health, 2501 Crosspark Rd, Coralville, IA, 52241, United StatesJhalka Kadariya - Kent State University, College of Public Health, Department of Biostatistics, Environmental Health Sciences and Epidemiology, 750Hilltop Drive, Kent, OH, 44242, United StatesMegan K Quick - Department of Epidemiology, University of Iowa College of Public Health, 145N. Riverside Drive, Iowa City, IA, 52242, United States; Center for Emerging Infectious Diseases, University of Iowa College of Public Health, 2501 Crosspark Rd, Coralville, IA, 52241, United StatesSarah Farina - Department of Epidemiology, University of Iowa College of Public Health, 145N. Riverside Drive, Iowa City, IA, 52242, United States; Center for Emerging Infectious Diseases, University of Iowa College of Public Health, 2501 Crosspark Rd, Coralville, IA, 52241, United StatesAshley O' Brien - Department of Epidemiology, University of Iowa College of Public Health, 145N. Riverside Drive, Iowa City, IA, 52242, United States; Center for Emerging Infectious Diseases, University of Iowa College of Public Health, 2501 Crosspark Rd, Coralville, IA, 52241, United StatesRajeshwari Nair - Department of Epidemiology, University of Iowa College of Public Health, 145N. Riverside Drive, Iowa City, IA, 52242, United States; Center for Emerging Infectious Diseases, University of Iowa College of Public Health, 2501 Crosspark Rd, Coralville, IA, 52241, United StatesAmos Nworie - Department of Epidemiology, University of Iowa College of Public Health, 145N. Riverside Drive, Iowa City, IA, 52242, United States; Center for Emerging Infectious Diseases, University of Iowa College of Public Health, 2501 Crosspark Rd, Coralville, IA, 52241, United StatesBlake Hanson - Department of Epidemiology, University of Iowa College of Public Health, 145N. Riverside Drive, Iowa City, IA, 52242, United States; Center for Emerging Infectious Diseases, University of Iowa College of Public Health, 2501 Crosspark Rd, Coralville, IA, 52241, United StatesAshley Kates - Department of Epidemiology, University of Iowa College of Public Health, 145N. Riverside Drive, Iowa City, IA, 52242, United States; Center for Emerging Infectious Diseases, University of Iowa College of Public Health, 2501 Crosspark Rd, Coralville, IA, 52241, United StatesShylo Wardyn - Department of Epidemiology, University of Iowa College of Public Health, 145N. Riverside Drive, Iowa City, IA, 52242, United States; Center for Emerging Infectious Diseases, University of Iowa College of Public Health, 2501 Crosspark Rd, Coralville, IA, 52241, United StatesTara C Smith - Department of Epidemiology, University of Iowa College of Public Health, 145N. Riverside Drive, Iowa City, IA, 52242, United States; Center for Emerging Infectious Diseases, University of Iowa College of Public Health, 2501 Crosspark Rd, Coralville, IA, 52241, United States; Kent State University, College of Public Health, Department of Biostatistics, Environmental Health Sciences and Epidemiology, 750Hilltop Drive, Kent, OH, 44242, United States. Electronic address: tsmit176@kent.edu
- Resource Type
- Journal article
- Publication Details
- Food microbiology, Vol.65, pp.122-129
- DOI
- 10.1016/j.fm.2017.01.015
- PMID
- 28399994
- ISSN
- 0740-0020
- eISSN
- 1095-9998
- Grant note
- name: USDA National Institute of Food and AgricultureUSDA National Institute of Food and Agriculture (TCS), award: 2011-67005-30337
- Language
- English
- Date published
- 08/2017
- Academic Unit
- Epidemiology; General Internal Medicine; Otolaryngology; Internal Medicine
- Record Identifier
- 9984094323802771
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