Journal article
Prevalence of Genes of OXA-23 Carbapenemase and AdeABC Efflux Pump Associated with Multidrug Resistance of Acinetobacter baumannii Isolates in the ICU of a Comprehensive Hospital of Northwestern China
International journal of environmental research and public health, Vol.12(8), pp.10079-10092
08/21/2015
DOI: 10.3390/ijerph120810079
PMCID: PMC4555330
PMID: 26308027
Abstract
The objective of this study was to explore the molecular epidemiology and the genetic support of clinical multidrug resistant (MDR) Acinetobacter baumannii (A. baumannii) isolates in an ICU ward of a comprehensive hospital. A total of 102 non-duplicate drug-resistant A. baumannii isolates were identified and 93 (91.1%) of them were MDR strains. Molecular analysis demonstrated that carbapenemase genes blaOXA-23 and blaOXA-51 were presented in all 93 MDR isolates (100%), but other carbapenemase genes, including blaOXA-24, blaOXA-58, blaIMP-1, blaIMP-4, blaSIM, and blaVIM genes were completely absent in all isolates. In addition, genes of AdeABC efflux system were detected in 88.2% (90/102) isolates. Interestingly, an addition to efflux pump inhibitor, reserpine could significantly enhance the susceptibility of MDR isolates to moxifloxacin, cefotaxime, and imipenem (p < 0.01). Clonal relationship analysis further grouped these clinical drug-resistant isolates into nine clusters, and the MDR strains were mainly in clusters A, B, C, and D, which include 16, 13, 25, and 15 isolates, respectively. This study demonstrated that clinical isolates carrying carbapenemase-encoding genes blaOXA-23 and AdeABC efflux pump genes are the main prevalent MDR A. baumannii, and the co-expression of oxacillinase and efflux pump proteins are thus considered to be the important reason for the prevalence of this organism in the ICU of this hospital.
Details
- Title: Subtitle
- Prevalence of Genes of OXA-23 Carbapenemase and AdeABC Efflux Pump Associated with Multidrug Resistance of Acinetobacter baumannii Isolates in the ICU of a Comprehensive Hospital of Northwestern China
- Creators
- Wei Jia - Ningxia Medical UniversityCaiyun Li - Ningxia Medical UniversityHaiyun Zhang - Ningxia Medical UniversityGang Li - Ningxia Medical UniversityXiaoming Liu - Ningxia Key laboratory of Clinical and Pathogenic Microbiology, Yinchuan, Ningxia 750004, China.Jun Wei - Ningxia Medical University
- Resource Type
- Journal article
- Publication Details
- International journal of environmental research and public health, Vol.12(8), pp.10079-10092
- DOI
- 10.3390/ijerph120810079
- PMID
- 26308027
- PMCID
- PMC4555330
- ISSN
- 1661-7827
- eISSN
- 1660-4601
- Language
- English
- Date published
- 08/21/2015
- Academic Unit
- Anatomy and Cell Biology
- Record Identifier
- 9984288756002771
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