Journal article
Prevalence of hepatitis C virus infection among human immunodeficiency virus-1-infected pregnant women in Malawi: The BAN study
Journal of clinical virology, Vol.54(4), pp.318-320
08/01/2012
DOI: 10.1016/j.jcv.2012.05.003
PMCID: PMC3652577
PMID: 22658797
Abstract
In Sub-Saharan Africa, prevalence estimates of hepatitis C virus (HCV) vary widely.
To assess the prevalence of HCV infection among HIV-infected, pregnant women screened for a large clinical trial in Lilongwe, Malawi.
Plasma from 2041 HIV-infected, pregnant women was screened for anti-HCV IgG using a chemiluminiscent immunometric assay (CIA). Specimens with a signal-cut-off ratio≥1.00 were considered reactive and those with S/Co ratio<1.00 non-reactive. All CIA-reactive specimens were tested by a recombinant immunoblot assay (RIBA) for anti-HCV and by PCR for HCV RNA.
Of 2041 specimens, 110 (5.3%, 95% CI: 4.5–6.5%) were CIA reactive. Of the 109 CIA reactive specimens available for RIBA testing, 2 (1.8%) were positive, 28 (25.7%) were indeterminate, and 79 (72.5%) were negative. All CIA-reactive specimens were HCV RNA negative (n=110). The estimated HCV prevalence based on the screening assay alone was 5.3%; based on supplemental RIBA testing, the status of HCV infection remained indeterminate in 1.4% (28/2040, 95% CI: 0.1–2.0) and the prevalence of confirmed HCV infections was 0.1% (2/2040, 95% CI: 0–0.4%).
HCV seroprevalence among HIV-infected, pregnant women in Malawi confirmed by supplemental RIBA HCV 3.0 is low (0.1%); CIA showed a high false-reactivity rate in this population.
Details
- Title: Subtitle
- Prevalence of hepatitis C virus infection among human immunodeficiency virus-1-infected pregnant women in Malawi: The BAN study
- Creators
- Charles S. Chasela - University of the WitwatersrandPatrick Wall - University College DublinJan Drobeniuc - Centers for Disease Control and PreventionCaroline C. King - Centers for Disease Control and PreventionEyasu Teshale - Centers for Disease Control and PreventionMina C. Hosseinipour - UNC Project, Lilongwe, MalawiSascha R. Ellington - Centers for Disease Control and PreventionMary Codd - University College DublinDenise J. Jamieson - Centers for Disease Control and PreventionRodney J. Knight - Principia CollegePatricia Fitzpatrick - University College DublinAthena P. Kourtis - Centers for Disease Control and PreventionIrving F. Hoffman - University of North Carolina at Chapel HillDumbani Kayira - UNC Project, Lilongwe, MalawiNoel Mumba - UNC Project, Lilongwe, MalawiDeborah D. Kamwendo - UNC Project, Lilongwe, MalawiFrancis Martinson - UNC Project, Lilongwe, MalawiWilliam Powderly - University College DublinCharles van der Horst - University of North Carolina at Chapel HillSaleem Kamili - Centers for Disease Control and PreventionBAN Study team
- Resource Type
- Journal article
- Publication Details
- Journal of clinical virology, Vol.54(4), pp.318-320
- DOI
- 10.1016/j.jcv.2012.05.003
- PMID
- 22658797
- PMCID
- PMC3652577
- NLM abbreviation
- J Clin Virol
- ISSN
- 1386-6532
- eISSN
- 1873-5967
- Publisher
- Elsevier B.V
- Language
- English
- Date published
- 08/01/2012
- Academic Unit
- Obstetrics and Gynecology; VPMA - Administration
- Record Identifier
- 9984446458502771
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