Journal article
Prevention of lipid peroxidation does not prevent oxidant-induced myocardial contractile dysfunction
American journal of physiology. Heart and circulatory physiology, Vol.267(6), pp.H2371-H2377
12/01/1994
DOI: 10.1152/ajpheart.1994.267.6.H2371
PMID: 7810737
Abstract
We tested whether, with exposure to an extraneous iron-catalyzed free radical-generating system, prevention of lipid peroxidation with U74006F, a 21-aminosteroid, could also prevent myocardial contractile dysfunction. Rabbits received either U74006F (10 mg/kg iv) or vehicle (V). Thirty minutes later the hearts were excised and perfused by a non-recirculating Langendorff technique. Six U74006F- and six V-treated hearts were exposed for 7.5 min to a .OH-generating system (H2O2 and Fe(2+)-ADP chelate). Myocardial lipid peroxides were measured by glutathione peroxidase-catalyzed oxidation of exogenous glutathione. With exposure to .OH, cytosolic lipid peroxide levels were increased threefold in V-treated hearts, but there was no increase in U74006F-treated hearts. After 30 min of recovery, developed pressure and maximum first derivative of left ventricular pressure were greater in U74006F-treated hearts than in V-treated hearts but were still 50 and 44% of levels in saline hearts, respectively. Coronary flow was markedly reduced after exposure to free radicals and was only slightly less depressed when U74006F was administered. When coronary flow following oxidant exposure was increased by nitroglycerin, U74006F again only modestly improved systolic function. Thus, although U74006F blocked lipid peroxidation, it only slightly improved the ventricular dysfunction caused by .OH. Therefore, factors other than lipid peroxidation play a major role in oxidant-induced myocardial stunning.
Details
- Title: Subtitle
- Prevention of lipid peroxidation does not prevent oxidant-induced myocardial contractile dysfunction
- Creators
- Yinong Kong - University of Colorado HealthEdward J Lesnefsky - University of Colorado HealthJian Ye - University of Colorado HealthLawrence D Horwitz - University of Colorado Health
- Resource Type
- Journal article
- Publication Details
- American journal of physiology. Heart and circulatory physiology, Vol.267(6), pp.H2371-H2377
- DOI
- 10.1152/ajpheart.1994.267.6.H2371
- PMID
- 7810737
- ISSN
- 0363-6135
- eISSN
- 1522-1539
- Language
- English
- Date published
- 12/01/1994
- Academic Unit
- Cardiovascular Medicine; Internal Medicine
- Record Identifier
- 9984656538802771
Metrics
6 Record Views