Journal article
Previously hidden chromosome aberrations in T(12;15)-positive BALB/c plasmacytomas uncovered by multicolor spectral karyotyping
Cancer research (Chicago, Ill.), Vol.57(20), pp.4585-4592
1997
PMID: 9377573
Abstract
The majority of BALB/c mouse plasmacytomas harbor a balanced T(12;15) chromosomal translocation deregulating the expression of the proto-oncogene c-myc. Recent evidence suggests that the T(12;15) is an initiating tumorigenic mutation that occurs in early plasmacytoma precursor cells. However, the possible contribution of additional chromosomal aberrations to the progression of plasmacytoma development has been largely ignored. Here we use multicolor spectral karyotyping (SKY) to evaluate 10 established BALB/c plasmacytomas in which the T(12;15) had been previously detected by G banding. SKY readily confirmed the presence of this translocation in all of these tumors and in three plasmacytomas newly identified secondary cytogenetic changes of the c-myc-deregulating chromosome (Chr) T(12;15). In addition, numerous previously unknown aberrations were found to be scattered throughout the genome, which was interpreted to reflect the general genomic instability of plasmacytomas. Instability of this sort was not uniform, however, because only half of the tumors were heavily rearranged. Seven apparent hot spots of chromosomal rearrangements (40% incidence) were identified and mapped to Chrs 1B, 1G-H, 2G-H1, 4C7-D2, 12D, 14C-D2, and XE-F1. Two of these regions, Chr 1B and Chr 4C7-D2, are suspected to harbor plasmacytoma susceptibility loci; Pctr1 and Pctr2 on Chr 4C7-D2 and as yet unnamed loci on Chr 1B. These results suggest that secondary chromosomal rearrangements contribute to plasmacytoma progression in BALB/c mice. To evaluate the biological significance of these rearrangements, SKY will be used in follow-up experiments to search for the presence of recurrent and/or consistent secondary cytogenetic aberrations in primary BALB/c plasmacytomas.
Details
- Title: Subtitle
- Previously hidden chromosome aberrations in T(12;15)-positive BALB/c plasmacytomas uncovered by multicolor spectral karyotyping
- Creators
- Allen E Coleman - Laboratory of Genetics, Division of Basic Sciences, National Cancer Institute, NIH, Bethesda, Maryland 20892-4255, United StatesEvelin Schröck - Genome Technology Branch, National Human Genome Research Institute, NIH, Bethesda, Maryland 20892-4470, United StatesZoë Weaver - Genome Technology Branch, National Human Genome Research Institute, NIH, Bethesda, Maryland 20892-4470, United StatesStan Du Manoir - Genome Technology Branch, National Human Genome Research Institute, NIH, Bethesda, Maryland 20892-4470, United StatesFangteng Yang - Department of Pathology, Cambridge University, Cambridge CB2 1QP, United KingdomMalcolm A Ferguson-Smith - Department of Pathology, Cambridge University, Cambridge CB2 1QP, United KingdomThomas Ried - Genome Technology Branch, National Human Genome Research Institute, NIH, Bethesda, Maryland 20892-4470, United StatesSiegfried Janz - Laboratory of Genetics, Division of Basic Sciences, National Cancer Institute, NIH, Bethesda, Maryland 20892-4255, United States
- Resource Type
- Journal article
- Publication Details
- Cancer research (Chicago, Ill.), Vol.57(20), pp.4585-4592
- Publisher
- American Association for Cancer Research
- PMID
- 9377573
- ISSN
- 0008-5472
- eISSN
- 1538-7445
- Language
- English
- Date published
- 1997
- Academic Unit
- Pathology
- Record Identifier
- 9984083845902771
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