Journal article
Prickle1 is necessary for the caudal migration of murine facial branchiomotor neurons
Cell and tissue research, Vol.357(3), pp.549-561
09/2014
DOI: 10.1007/s00441-014-1925-6
PMCID: PMC4149827
PMID: 24927917
Abstract
Facial branchiomotor neurons (FBMs) of vertebrates typically develop in rhombomere 4 (r4) and in mammals and several other vertebrate taxa, migrate caudally into r6 and subsequently laterally and ventrally to the pial surface. How similar or dissimilar these migratory processes between species are at a molecular level remains unclear. In zebrafish and mouse, mutations in certain PCP genes disrupt normal caudal migration of FBMs. Zebrafish
prickle1a
(
prickle-like 1a
) and
prickle1b
, two orthologs of
Prickle1
, act non-cell autonomously and cell-autonomously, respectively, to regulate FBM migration. Here we show that in
Prickle1
C251X/C251X
mice, which have reduced
Prickle1
expression, the caudal migration of FBMs is affected. Most FBM neurons do not migrate caudally along the floor plate. However, some neurons perform limited caudal migration such that the neurons eventually lie near the pial surface from r4 to anterior r6. FBMs in
Prickle1
C251X/C251X
mice survive until P0 and form an ectopic nucleus dorsal to the olivo-cochlear efferents of r4.
Ror2,
which modifies the PCP pathway in other systems, is expressed by the migrating mouse FBMs, but is not required for FBM caudal migration. Our results suggest that in mice, Prickle1 is part of a molecular mechanism that regulates FBM caudal migration and separates the FBM and the olivo-cochlear efferents. This defective caudal migration of FBMs in
Prickle1C251X
mutants resembles
Vangl2
mutant defects. In contrast to other developing systems that show similar defects in Prickle1, Wnt5a and Ror2, Wnt5a and Ror2 only have limited or no effect on FBM caudal migration.
Details
- Title: Subtitle
- Prickle1 is necessary for the caudal migration of murine facial branchiomotor neurons
- Creators
- Tian Yang - University of Iowa. Department of Biology, Iowa City, IA, USA, 52242Alexander G Bassuk - University of Iowa, Department of Pediatrics, Iowa City, IA, USA, 52242Sigmar Stricker - Max Planck Institute for Molecular Genetics, Berlin, Germany, 14195Bernd Fritzsch - University of Iowa. Department of Biology, Iowa City, IA, USA, 52242
- Resource Type
- Journal article
- Publication Details
- Cell and tissue research, Vol.357(3), pp.549-561
- DOI
- 10.1007/s00441-014-1925-6
- PMID
- 24927917
- PMCID
- PMC4149827
- NLM abbreviation
- Cell Tissue Res
- ISSN
- 0302-766X
- eISSN
- 1432-0878
- Language
- English
- Date published
- 09/2014
- Academic Unit
- Neurology; Stead Family Department of Pediatrics; Iowa Neuroscience Institute; Biology; Craniofacial Anomalies Research Center; Neurology (Pediatrics)
- Record Identifier
- 9984020763702771
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