Prickle1 regulates neurite outgrowth of apical spiral ganglion neurons but not hair cell polarity in the murine cochlea

Tian Yang; Jennifer Kersigo; Shu Wu; Bernd Fritzsch; Alexander G Bassuk

doi:10.1371/journal.pone.0183773

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Prickle1 regulates neurite outgrowth of apical spiral ganglion neurons but not hair cell polarity in the murine cochlea

Journal article

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Prickle1 regulates neurite outgrowth of apical spiral ganglion neurons but not hair cell polarity in the murine cochlea

Tian Yang, Jennifer Kersigo, Shu Wu, Bernd Fritzsch and Alexander G Bassuk

PloS one, Vol.12(8), pp.e0183773-e0183773

2017

DOI: 10.1371/journal.pone.0183773

PMCID: PMC5570324

PMID: 28837644

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Abstract

In the mammalian organ of Corti (OC), the stereocilia on the apical surface of hair cells (HCs) are uniformly organized in a neural to abneural axis (or medial-laterally). This organization is regulated by planar cell polarity (PCP) signaling. Mutations of PCP genes, such as Vangl2, Dvl1/2, Celsr1, and Fzd3/6, affect the formation of HC orientation to varying degrees. Prickle1 is a PCP signaling gene that belongs to the prickle / espinas / testin family. Prickle1 protein is shown to be asymmetrically localized in the HCs of the OC, and this asymmetric localization is associated with loss of PCP in Smurf mutants, implying that Prickle1 is involved in HC PCP development in the OC. A follow-up study found no PCP polarity defects after loss of Prickle1 (Prickle1-/-) in the cochlea. We show here strong Prickle1 mRNA expression in the spiral ganglion by in situ hybridization and β-Gal staining, and weak expression in the OC by β-Gal staining. Consistent with this limited expression in the OC, cochlear HC PCP is unaffected in either Prickle1C251X/C251X mice or Prickle1f/f; Pax2-cre conditional null mice. Meanwhile, type II afferents of apical spiral ganglion neurons (SGN) innervating outer hair cells (OHC) have unusual neurite growth. In addition, afferents from the apex show unusual collaterals in the cochlear nuclei that overlap with basal turn afferents. Our findings argue against the role of Prickle1 in regulating hair cell polarity in the cochlea. Instead, Prickle1 regulates the polarity-related growth of distal and central processes of apical SGNs.

Hair Cells, Auditory - cytology

Microscopy, Electron, Scanning

Neurites

Mice, Inbred C57BL

Mice, Transgenic

Neurons - cytology

Cochlea - cytology

Spiral Ganglion - cytology

Adaptor Proteins, Signal Transducing - physiology

Animals

Cell Polarity - physiology

LIM Domain Proteins - physiology

Mice

Details

Title: Subtitle: Prickle1 regulates neurite outgrowth of apical spiral ganglion neurons but not hair cell polarity in the murine cochlea
Creators: Tian Yang - Department of Biology, University of Iowa, Iowa City, Iowa, United States of America
Jennifer Kersigo - Department of Biology, University of Iowa, Iowa City, Iowa, United States of America
Shu Wu - Department of Pediatrics, University of Iowa, Iowa City, Iowa, United States of America
Bernd Fritzsch - Department of Biology, University of Iowa, Iowa City, Iowa, United States of America
Alexander G Bassuk - Department of Pediatrics, University of Iowa, Iowa City, Iowa, United States of America
Resource Type: Journal article
Publication Details: PloS one, Vol.12(8), pp.e0183773-e0183773
DOI: 10.1371/journal.pone.0183773
PMID: 28837644
PMCID: PMC5570324
NLM abbreviation: PLoS One
ISSN: 1932-6203
eISSN: 1932-6203
Publisher: Public Library of Science; United States
Grant note: R01 DC005590 / NIDCD NIH HHS R01 NS064159 / NINDS NIH HHS P30 DC010362 / NIDCD NIH HHS
Language: English
Date published: 2017
Academic Unit: Neurology; Stead Family Department of Pediatrics; Iowa Neuroscience Institute; Biology; Craniofacial Anomalies Research Center; Neurology (Pediatrics); Internal Medicine
Record Identifier: 9984070571102771

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