Primary Culture of Human Vestibular Schwannomas

Nathan M Schularick; J. Jason Clark; Marlan R Hansen

doi:10.3791/51093

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Primary Culture of Human Vestibular Schwannomas

Journal article

Open access

Peer reviewed

Primary Culture of Human Vestibular Schwannomas

Nathan M Schularick, J. Jason Clark and Marlan R Hansen

Journal of Visualized Experiments, Vol.89, e51093

2014

DOI: 10.3791/51093

PMCID: PMC4220898

PMID: 25079666

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https://doi.org/10.3791/51093View

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Abstract

Vestibular schwannomas (VSs) represent Schwann cell (SC) tumors of the vestibular nerve, compromising 10% of all intracranial neoplasms. VSs occur in either sporadic or familial (neurofibromatosis type 2, NF2) forms, both associated with inactivating defects in the NF2 tumor suppressor gene. Treatment for VSs is generally surgical resection or radiosurgery, however the morbidity of such procedures has driven investigations into less invasive treatments. Historically, lack of access to fresh tissue specimens and the fact that schwannoma cells are not immortalized have significantly hampered the use of primary cultures for investigation of schwannoma tumorigenesis. To overcome the limited supply of primary cultures, the immortalized HEI193 VS cell line was generated by transduction with HPV E6 and E7 oncogenes. This oncogenic transduction introduced significant molecular and phenotypic alterations to the cells, which limit their use as a model for human schwannoma tumors. We therefore illustrate a simplified, reproducible protocol for culture of primary human VS cells. This easily mastered technique allows for molecular and cellular investigations that more accurately recapitulate the complexity of VS disease.

Medicine

Details

Title: Subtitle: Primary Culture of Human Vestibular Schwannomas
Creators: Nathan M Schularick - Department of Otolaryngology-Head and Neck, University of Iowa Hospitals and Clinics
J. Jason Clark - Department of Otolaryngology-Head and Neck, University of Iowa Hospitals and Clinics
Marlan R Hansen - Department of Otolaryngology-Head and Neck, University of Iowa Hospitals and Clinics
Resource Type: Journal article
Publication Details: Journal of Visualized Experiments, Vol.89, e51093
DOI: 10.3791/51093
PMID: 25079666
PMCID: PMC4220898
NLM abbreviation: J Vis Exp
ISSN: 1940-087X
eISSN: 1940-087X
Publisher: MyJove Corporation
Language: English
Date published: 2014
Academic Unit: Molecular Physiology and Biophysics; Neurosurgery; Otolaryngology
Record Identifier: 9984006370102771

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