Primary bone diffuse large B-cell lymphoma: clinicopathologic study of 21 cases and review of literature

Sharathkumar Bhagavathi; Mark A Micale; Kimberly Les; Jon D Wilson; Michele L Wiggins; Kai Fu

doi:10.1097/PAS.0b013e3181b314ce

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Primary bone diffuse large B-cell lymphoma: clinicopathologic study of 21 cases and review of literature

Journal article

Peer reviewed

Primary bone diffuse large B-cell lymphoma: clinicopathologic study of 21 cases and review of literature

Sharathkumar Bhagavathi, Mark A Micale, Kimberly Les, Jon D Wilson, Michele L Wiggins and Kai Fu

The American journal of surgical pathology, Vol.33(10), pp.1463-1469

10/2009

DOI: 10.1097/PAS.0b013e3181b314ce

PMID: 19675454

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Abstract

Primary bone diffuse large B-cell lymphomas (PB-DLBCL) are uncommon extranodal lymphomas. Herein, we report the clinical, pathologic, immunohistochemical, and molecular features of 21 cases of PB-DLBCL. The mean age of the patients was 54 years (range: 13 to 85 y). The male and female ratio was 1.6:1. The tumors consisted of diffuse sheets of large atypical cells or a polymorphous mixture of small-to-large cells with large multilobated nuclei, fine chromatin, and inconspicuous to prominent nucleoli. Twelve (57%) cases were non-germinal center B (GCB) and 9 (43%) were GCB subtype based on immunohistochemical classification. B-cell lymphomas (BCL)-2 was positive in 17/21 (81%), TP53 in 11/21 (52%) positive and the mean MIB-1 index was 57%. Polymerase chain reaction showed 10 cases with immunoglobulin heavy-chain (IGH) and 4 cases with IGH/BCL-2 gene rearrangement. The fluorescence in-situ hybridization analyses showed 14% of cases with BCL-6, 19% of cases with BCL-2, and 9% of cases with C-MYC gene rearrangement. Age <60 years and complete response to initial treatment were significant predictors of survival outcome (P< or =0.05). Even though no association was observed between the subtype of PB-DLBCL (GCB vs. non-GCB), BCL2, TP53, MIB1 index and overall survival (P>0.05), due to small sample size, and variability in treatment received, this analysis may be interpreted with caution.

Immunohistochemistry

Bone Neoplasms - mortality

Translocation, Genetic

Prognosis

Age Factors

Humans

Middle Aged

Male

Genes, myc

Bone Neoplasms - pathology

Tumor Suppressor Protein p53 - genetics

Young Adult

Polymerase Chain Reaction

Aged, 80 and over

Adult

Female

Bone Neoplasms - genetics

Lymphoma, Large B-Cell, Diffuse - pathology

Biomarkers, Tumor - analysis

In Situ Hybridization, Fluorescence

Genes, bcl-2

DNA-Binding Proteins - genetics

Lymphoma, Large B-Cell, Diffuse - mortality

Ki-67 Antigen - genetics

Adolescent

Aged

Lymphoma, Large B-Cell, Diffuse - genetics

Proto-Oncogene Proteins c-bcl-6

Details

Title: Subtitle: Primary bone diffuse large B-cell lymphoma: clinicopathologic study of 21 cases and review of literature
Creators: Sharathkumar Bhagavathi - Department of Pathology, Indiana University, Indianapolis, IN, USA
Mark A Micale
Kimberly Les
Jon D Wilson
Michele L Wiggins
Kai Fu
Resource Type: Journal article
Publication Details: The American journal of surgical pathology, Vol.33(10), pp.1463-1469
Publisher: United States
DOI: 10.1097/PAS.0b013e3181b314ce
PMID: 19675454
ISSN: 0147-5185
eISSN: 1532-0979
Language: English
Date published: 10/2009
Academic Unit: Pathology
Record Identifier: 9984047765002771

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