Primary structure and topological analysis of a skeletal muscle-specific junctional sarcoplasmic reticulum glycoprotein (triadin)

C Michael Knudson; Kristin K Stang; Carolyn R Moomaw; Clive A Slaughter; Kevin P Campbell

doi:10.1016/S0021-9258(18)31437-6

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Primary structure and topological analysis of a skeletal muscle-specific junctional sarcoplasmic reticulum glycoprotein (triadin)

Journal article

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Primary structure and topological analysis of a skeletal muscle-specific junctional sarcoplasmic reticulum glycoprotein (triadin)

C Michael Knudson, Kristin K Stang, Carolyn R Moomaw, Clive A Slaughter and Kevin P Campbell

The Journal of biological chemistry, Vol.268(17), pp.12646-12654

1993

DOI: 10.1016/S0021-9258(18)31437-6

PMID: 7685347

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Abstract

The primary amino acid sequence for a highly abundant junctional sarcoplasmic reticulum glycoprotein (triadin) has been deduced from the cDNA sequence. Based on both biochemical analysis and the predicted amino acid sequence we suggest that this protein is an intrinsic membrane glycoprotein containing a single transmembrane domain that separates the protein into cytoplasmic and luminal domains. The cytoplasmic domain is proposed to contain the amino-terminal 47 amino acids. The remainder of the protein including the carboxyl terminus is proposed to be found within the lumen of the sarcoplasmic reticulum and contains an extremely high concentration of basic residues. Protease analysis of intact triads was consistent with the topological predictions. Western and Northern blots suggest that the protein is specifically expressed in skeletal muscle and not cardiac muscle or brain. The abundance and localization of this protein suggest that it plays an important regulatory or structural role in excitation-contraction coupling in skeletal muscle.

Fundamental and applied biological sciences. Psychology

Proteins

Biological and medical sciences

Glycoproteins

Analytical, structural and metabolic biochemistry

Details

Title: Subtitle: Primary structure and topological analysis of a skeletal muscle-specific junctional sarcoplasmic reticulum glycoprotein (triadin)
Creators: C Michael Knudson - Univ. Iowa coll. medicine, Howard Hughes medical inst., dep. physiology biophysics, Iowa City IA 52242, United States
Kristin K Stang - Univ. Iowa coll. medicine, Howard Hughes medical inst., dep. physiology biophysics, Iowa City IA 52242, United States
Carolyn R Moomaw - Univ. Iowa coll. medicine, Howard Hughes medical inst., dep. physiology biophysics, Iowa City IA 52242, United States
Clive A Slaughter - Univ. Iowa coll. medicine, Howard Hughes medical inst., dep. physiology biophysics, Iowa City IA 52242, United States
Kevin P Campbell - Univ. Iowa coll. medicine, Howard Hughes medical inst., dep. physiology biophysics, Iowa City IA 52242, United States
Resource Type: Journal article
Publication Details: The Journal of biological chemistry, Vol.268(17), pp.12646-12654
DOI: 10.1016/S0021-9258(18)31437-6
PMID: 7685347
NLM abbreviation: J Biol Chem
ISSN: 0021-9258
eISSN: 1083-351X
Publisher: American Society for Biochemistry and Molecular Biology; Bethesda, MD
Language: English
Date published: 1993
Academic Unit: Neurology; Molecular Physiology and Biophysics; Pathology; Iowa Neuroscience Institute; Radiation Oncology
Record Identifier: 9984046825102771

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