Primary structure of the mouse sperm receptor polypeptide determined by genomic cloning

R A Kinloch; R J Roller; C M Fimiani; D A Wassarman; P M Wassarman

doi:10.1073/pnas.85.17.6409

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Primary structure of the mouse sperm receptor polypeptide determined by genomic cloning

Journal article

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Primary structure of the mouse sperm receptor polypeptide determined by genomic cloning

R A Kinloch, R J Roller, C M Fimiani, D A Wassarman and P M Wassarman

Proceedings of the National Academy of Sciences - PNAS, Vol.85(17), pp.6409-6413

09/1988

DOI: 10.1073/pnas.85.17.6409

PMID: 2842770

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Abstract

The mouse sperm receptor, a glycoprotein called ZP3, is synthesized and secreted by growing oocytes. It is present in more than a billion copies in the unfertilized egg's extracellular coat, or zona pellucida. We have cloned and characterized a region of the mouse (CD-1) genome that spans 10 kilobases of the ZP3 locus. The genomic clones described encompass the entire ZP3 coding region, which contains eight exons. The exons were identified, mapped, and sequenced, yielding the entire primary structure of the ZP3 polypeptide chain (424 amino acids; Mr, 46,300), which includes a 22-amino acid signal sequence. In addition, sequencing of genomic clones has revealed some unusual features of ZP3 mRNA and a region just downstream of the ZP3 gene.

Details

Title: Subtitle: Primary structure of the mouse sperm receptor polypeptide determined by genomic cloning
Creators: R A Kinloch - Department of Cell and Developmental Biology, Roche Research Center, Nutley, NJ 07110
R J Roller - Department of Cell and Developmental Biology, Roche Research Center, Nutley, NJ 07110
C M Fimiani - Department of Cell and Developmental Biology, Roche Research Center, Nutley, NJ 07110
D A Wassarman - Department of Cell and Developmental Biology, Roche Research Center, Nutley, NJ 07110
P M Wassarman - Department of Cell and Developmental Biology, Roche Research Center, Nutley, NJ 07110
Resource Type: Journal article
Publication Details: Proceedings of the National Academy of Sciences - PNAS, Vol.85(17), pp.6409-6413
DOI: 10.1073/pnas.85.17.6409
PMID: 2842770
NLM abbreviation: Proc Natl Acad Sci U S A
ISSN: 0027-8424
eISSN: 1091-6490
Publisher: National Academy of Sciences
Language: English
Date published: 09/1988
Academic Unit: Microbiology and Immunology
Record Identifier: 9984001118402771

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