Journal article
Primary structure of the γ subunit of the DHP-sensitive calcium channel from skeletal muscle
Science (American Association for the Advancement of Science), Vol.248(4954), pp.490-492
1990
DOI: 10.1126/science.2158672
PMID: 2158672
Abstract
Affinity-purified, polyclonal antibodies to the gamma subunit of the dihydropyridine (DHP)-sensitive, voltage-dependent calcium channel have been used to isolate complementary DNAs to the rabbit skeletal muscle protein from an expression library. The deduced primary structure indicates that the gamma subunit is a 25,058-dalton protein that contains four transmembrane domains and two N-linked glycosylation sites, consistent with biochemical analyses showing that the gamma subunit is a glycosylated hydrophobic protein. Nucleic acid hybridization studies indicate that there is a 1200-nucleotide transcript in skeletal muscle but not in brain or heart. The gamma subunit may play a role in assembly, modulation, or the structure of the skeletal muscle calcium channe
Details
- Title: Subtitle
- Primary structure of the γ subunit of the DHP-sensitive calcium channel from skeletal muscle
- Creators
- Scott D Jay - Univ. Iowa coll. medicine, howard hughes inst., dep. physiology biophysics, Iowa City IA 52242, United StatesSteven B Ellis - Univ. Iowa coll. medicine, howard hughes inst., dep. physiology biophysics, Iowa City IA 52242, United StatesAnn F McCue - Univ. Iowa coll. medicine, howard hughes inst., dep. physiology biophysics, Iowa City IA 52242, United StatesMark E Williams - Univ. Iowa coll. medicine, howard hughes inst., dep. physiology biophysics, Iowa City IA 52242, United StatesThomas S Vedvick - Univ. Iowa coll. medicine, howard hughes inst., dep. physiology biophysics, Iowa City IA 52242, United StatesMichael M Harpold - Univ. Iowa coll. medicine, howard hughes inst., dep. physiology biophysics, Iowa City IA 52242, United StatesKevin P Campbell - Univ. Iowa coll. medicine, howard hughes inst., dep. physiology biophysics, Iowa City IA 52242, United States
- Resource Type
- Journal article
- Publication Details
- Science (American Association for the Advancement of Science), Vol.248(4954), pp.490-492
- Publisher
- American Association for the Advancement of Science; Washington, DC
- DOI
- 10.1126/science.2158672
- PMID
- 2158672
- ISSN
- 0036-8075
- eISSN
- 1095-9203
- Language
- English
- Date published
- 1990
- Academic Unit
- Neurology; Molecular Physiology and Biophysics; Iowa Neuroscience Institute
- Record Identifier
- 9984068384702771
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