Journal article
Primary tumor microbiomes predict distant metastasis of colorectal cancer
NPJ precision oncology, Vol.9(1), 405
2025
DOI: 10.1038/s41698-025-01188-x
PMCID: PMC12748615
PMID: 41286005
Abstract
Metastasis causes most cancer-related deaths in colorectal carcinoma (CRC), and microbiome markers may have prognostic value. We hypothesized that primary tumor microbiomes predict distant metastases. We analyzed 5-year metastasis-free survival (MFS) in a retrospective cohort of 900 ORIEN CRC tumor microbiomes (RNAseq). ORIEN findings were validated on an independent cohort using 16S rDNA sequencing and pathobiont-specific qPCR. Microbiome alpha diversity was higher in primary tumors than metastases and positively correlated with metastasis risk. Microbiome beta diversity distinguished primary vs. metastasis and predicted 5-year MFS. High primary tumor abundance of B. fragilis and low F. nucleatum were associated with short MFS. Enterobacteriaceae, including E. coli, were enriched in metastases. qPCR identified increased enterotoxigenic B. fragilis and pks + E. coli detection in CRC metastasizers. Microbial co-occurrence analysis identified a 3-species clique that predicts metastasis (OR 1.9 [1.4-2.6]). Results suggest that primary tumor microbiomes and specific pathobionts are precision markers for metastasis risk.
Details
- Title: Subtitle
- Primary tumor microbiomes predict distant metastasis of colorectal cancer
- Creators
- Bishal Parajuli - University of IowaVishal Midya - Icahn School of Medicine at Mount SinaiRyan Kiddle - University of DubuqueNicola De Jager - University of IowaShoshannah Eggers - University of IowaDaniel Spakowicz - The Ohio State UniversityRebecca Hoyd - The Ohio State UniversityBodour Salhia - University of Southern CaliforniaCarlos H F Chan - University of IowaMichelle L Churchman - Aster Insights, Hudson, FL, USARobert J Rounbehler - Aster Insights, Hudson, FL, USASong Yao - Roswell Park Comprehensive Cancer CenterMelanie R Rutkowski - University of VirginiaAhmad A Tarhini - Moffitt Cancer CenterDinesh Pal Mudaranthakam - The University of Kansas Cancer CenterAshiq Masood - Indiana University HealthTherese J Bocklage - University of KentuckyRobert W Lentz - University of Colorado DenverHassan Hatoum - University of Oklahoma Health Sciences CenterMmadili N Ilozumba - Huntsman Cancer InstituteSheetal Hardikar - Huntsman Cancer InstituteCornelia M Ulrich - Huntsman Cancer InstituteJune L Round - Huntsman Cancer InstituteGregory Riedlinger - Rutgers, The State University of New JerseyCraig D Shriver - Uniformed Services University of the Health SciencesDustin E Bosch - University of Iowa
- Resource Type
- Journal article
- Publication Details
- NPJ precision oncology, Vol.9(1), 405
- DOI
- 10.1038/s41698-025-01188-x
- PMID
- 41286005
- PMCID
- PMC12748615
- NLM abbreviation
- NPJ Precis Oncol
- ISSN
- 2397-768X
- eISSN
- 2397-768X
- Publisher
- Springer Nature
- Grant note
- K08AI159619 / National Institute of Allergy and Infectious Diseases R25CA273964 / NCI NIH HHS K01AG070310 / NIA NIH HHS RSG-23-1023205 / American Cancer Society IRG-21-141-46-IRG / American Cancer Society 1046611 / American Lung Association
- Language
- English
- Electronic publication date
- 11/24/2025
- Date published
- 2025
- Academic Unit
- Epidemiology; Pathology; Surgery; Radiation Oncology; Injury Prevention Research Center
- Record Identifier
- 9985035041002771
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