Journal article
Principles and approaches for reproducible scoring of tissue stains in research
Laboratory investigation, Vol.98(7), pp.844-855
07/2018
DOI: 10.1038/s41374-018-0057-0
PMID: 29849125
Abstract
Evaluation of tissues is a common and important aspect of translational research studies. Labeling techniques such as immunohistochemistry can stain cells/tissues to enhance identification of specific cell types, cellular activation states, and protein expression. While qualitative evaluation of labeled tissues has merit, use of semiquantitative and quantitative scoring approaches can greatly enhance the rigor of the tissue data. Adhering to key principles for reproducible scoring can enhance the quality and reproducibility of the tissue data so as to maximize its biological relevance and scientific impact.
Details
- Title: Subtitle
- Principles and approaches for reproducible scoring of tissue stains in research
- Creators
- David K Meyerholz - Department of Pathology, University of Iowa Carver College of Medicine, Iowa City, IA, USA. david-meyerholz@uiowa.eduAmanda P Beck - Department of Pathology, Albert Einstein College of Medicine, Bronx, NY, USA. amanda.beck@einstein.yu.edu
- Resource Type
- Journal article
- Publication Details
- Laboratory investigation, Vol.98(7), pp.844-855
- DOI
- 10.1038/s41374-018-0057-0
- PMID
- 29849125
- ISSN
- 0023-6837
- eISSN
- 1530-0307
- Grant note
- P30 DK054759 / NIDDK NIH HHS
- Language
- English
- Date published
- 07/2018
- Academic Unit
- Pathology
- Record Identifier
- 9984083291202771
Metrics
42 Record Views