Journal article
Principles for valid histopathologic scoring in research
Veterinary pathology, Vol.50(6), pp.1007-1015
11/2013
DOI: 10.1177/0300985813485099
PMCID: PMC3795863
PMID: 23558974
Abstract
Histopathologic scoring is a tool by which semi-quantitative data can be obtained from tissues. Initially, a thorough understanding of the experimental design, study objectives and methods are required to allow the pathologist to appropriately examine tissues and develop lesion scoring approaches. Many principles go into the development of a scoring system such as tissue examination, lesion identification, scoring definitions and consistency in interpretation. Masking (a.k.a. “blinding”) of the pathologist to experimental groups is often necessary to constrain bias and multiple mechanisms are available. Development of a tissue scoring system requires appreciation of the attributes and limitations of the data (e.g. nominal, ordinal, interval and ratio data) to be evaluated. Incidence, ordinal and rank methods of tissue scoring are demonstrated along with key principles for statistical analyses and reporting. Validation of a scoring system occurs through two principal measures: 1) validation of repeatability and 2) validation of tissue pathobiology. Understanding key principles of tissue scoring can help in the development and/or optimization of scoring systems so as to consistently yield meaningful and valid scoring data.
Details
- Title: Subtitle
- Principles for valid histopathologic scoring in research
- Creators
- Katherine N Gibson-CorleyAlicia K Olivier - Roy J. and Lucille A. Carver College of MedicineDavid K Meyerholz - Roy J. and Lucille A. Carver College of Medicine
- Resource Type
- Journal article
- Publication Details
- Veterinary pathology, Vol.50(6), pp.1007-1015
- DOI
- 10.1177/0300985813485099
- PMID
- 23558974
- PMCID
- PMC3795863
- ISSN
- 0300-9858
- eISSN
- 1544-2217
- Grant note
- R24 DK091211 || DK / National Institute of Diabetes and Digestive and Kidney Diseases : NIDDK P30 DK054759 || DK / National Institute of Diabetes and Digestive and Kidney Diseases : NIDDK P01 HL091842 || HL / National Heart, Lung, and Blood Institute : NHLBI P01 HL051670 || HL / National Heart, Lung, and Blood Institute : NHLBI
- Language
- English
- Date published
- 11/2013
- Academic Unit
- The University of Iowa Institute for Vision Research; Pathology; Ophthalmology and Visual Sciences
- Record Identifier
- 9984186423002771
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