Journal article
Probing Cell Deformability via Acoustically Actuated Bubbles
Small (Weinheim an der Bergstrasse, Germany), Vol.12(7), pp.902-910
02/17/2016
DOI: 10.1002/smll.201502220
PMCID: PMC4876965
PMID: 26715211
Abstract
A
n acoustically actuated, bubble-based technique is developed to investigate the deformability of cells suspended in microfluidic devices. A microsized bubble is generated by an optothermal effect near the targeted cells, which are suspended in a microfluidic chamber. Subsequently, acoustic actuation is employed to create localized acoustic streaming. In turn, the streaming flow results in hydrodynamic forces that deform the cells in situ. The deformability of the cells is indicative of their mechanical properties. The method in this study measures mechanical biomarkers from multiple cells in a single experiment, and it can be conveniently integrated with other bioanalysis and drug-screening platforms. Using this technique, the mean deformability of tens of HeLa, HEK, and HUVEC cells is measured to distinguish their mechanical properties. HeLa cells are deformed upon treatment with Cytochalasin. The technique also reveals the deformability of each subpopulation in a mixed, heterogeneous cell sample by the use of both fluorescent markers and mechanical biomarkers. The technique in this study, apart from being relevant to cell biology, will also enable biophysical cellular diagnosis.
Details
- Title: Subtitle
- Probing Cell Deformability via Acoustically Actuated Bubbles
- Creators
- Yuliang Xie - Pennsylvania State UniversityNitesh Nama - Pennsylvania State UniversityPeng Li - Pennsylvania State UniversityZhangming Mao - Pennsylvania State UniversityPo-Hsun Huang - Pennsylvania State UniversityChenglong Zhao - University of DaytonFrancesco Costanzo - Pennsylvania State UniversityTony Jun Huang - Pennsylvania State University
- Resource Type
- Journal article
- Publication Details
- Small (Weinheim an der Bergstrasse, Germany), Vol.12(7), pp.902-910
- DOI
- 10.1002/smll.201502220
- PMID
- 26715211
- PMCID
- PMC4876965
- NLM abbreviation
- Small
- ISSN
- 1613-6810
- eISSN
- 1613-6829
- Grant note
- DOI: 10.13039/100000002, name: National Institutes of Health, award: 1 R01 GM112048-01A1, 1R33EB019785-01; name: National Science Foundation, award: CBET-1438126, IDBR-1455658; name: Penn State Center for Nanoscale Science, award: DMR-1420620; name: National Nanotechnology Infrastructure Network
- Language
- English
- Date published
- 02/17/2016
- Academic Unit
- Roy J. Carver Department of Biomedical Engineering
- Record Identifier
- 9984197152202771
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