Probing rhodopsin–transducin interaction using Drosophila Rh1–bovine rhodopsin chimeras

Michael Natochin; Brandy Barren; Syed Tariq Ahmad; Joseph E O’Tousa; Nikolai O Artemyev

doi:10.1016/j.visres.2006.07.013

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Probing rhodopsin–transducin interaction using Drosophila Rh1–bovine rhodopsin chimeras

Journal article

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Probing rhodopsin–transducin interaction using Drosophila Rh1–bovine rhodopsin chimeras

Michael Natochin, Brandy Barren, Syed Tariq Ahmad, Joseph E O’Tousa and Nikolai O Artemyev

Vision research (Oxford), Vol.46(27), pp.4575-4581

2006

DOI: 10.1016/j.visres.2006.07.013

PMID: 16979689

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Abstract

Invertebrate and vertebrate rhodopsins share a low degree of homology and are coupled to G-proteins from different families. Here we explore the utility of fly-expressed chimeras between Drosophila rhodopsin Rh1 and bovine rhodopsin (Rho) to probe the interactions between the invertebrate and vertebrate visual pigments and their cognate G-proteins. Chimeric Rh1 pigments carrying individual substitutions of the cytoplasmic loops C2 and C3 and the C-terminus with the corresponding regions of Rho retained the ability to stimulate phototranduction in Drosophila, but failed to activate transducin. Surprisingly, chimeric Rho containing the Rh1 C-terminus was fully capable of transducin activation, indicating that the C-terminal domain of vertebrate rhodopsins is not essential for the functional coupling to transducin.

Transducin

GPCR

Rhodopsin

Phototransduction

Drosophila rhodopsin Rh1

G proteins

Details

Title: Subtitle: Probing rhodopsin–transducin interaction using Drosophila Rh1–bovine rhodopsin chimeras
Creators: Michael Natochin - Department of Physiology and Biophysics, University of Iowa College of Medicine, Iowa City, IA 52242, USA
Brandy Barren - Department of Physiology and Biophysics, University of Iowa College of Medicine, Iowa City, IA 52242, USA
Syed Tariq Ahmad - Department of Biological Sciences, University of Notre Dame, Notre Dame, IN 46556, USA
Joseph E O’Tousa - Department of Biological Sciences, University of Notre Dame, Notre Dame, IN 46556, USA
Nikolai O Artemyev - Department of Physiology and Biophysics, University of Iowa College of Medicine, Iowa City, IA 52242, USA
Resource Type: Journal article
Publication Details: Vision research (Oxford), Vol.46(27), pp.4575-4581
DOI: 10.1016/j.visres.2006.07.013
PMID: 16979689
NLM abbreviation: Vision Res
ISSN: 0042-6989
eISSN: 1878-5646
Publisher: Elsevier Ltd
Language: English
Date published: 2006
Academic Unit: Molecular Physiology and Biophysics; Iowa Neuroscience Institute; Ophthalmology and Visual Sciences
Record Identifier: 9984070946802771

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