Journal article
Probiotic domestication and engineering enable one-shot treatment for bladder mucosal repair
Biomaterials, Vol.318, 123123
07/2025
DOI: 10.1016/j.biomaterials.2025.123123
PMID: 39893782
Abstract
The bladder mucosa is an important blood-urine barrier in the human body, its destruction can lead to distressing cystitis. Traditional treatment approaches often require frequent catheterization and intravesical instillation of hyaluronic acid (HA), which greatly reduces patient compliance and therapeutic efficacy. Herein, we develop a probiotic-based one-shot therapy to repair bladder mucosa with improved convenience, efficacy, and biosafety. To this end, a high-biocompatible probiotic strain is engineered to secrete high-molecular-weight HA controlled by ultrasound stimulation. Meanwhile, a bacterium acclimation-inspired strategy to select bacterial cells targeting the site of bladder inflammation is also proposed. With just one-shot intravesical administration, these engineered bacteria can strongly adhere to the damaged bladder epithelium, continuously secrete HA, and stimulate the formation of protective living engineered materials on the bladder. Consequently, varying therapeutic efficacies in damaged murine model, such as reporting the site of inflammation within 28 days, accelerating mucosal repair (such as significantly increased expression of tight junction proteins occludin-1 and ZO-1), modulating innate immune reactions (such as pro-inflammatory factor levels decreased by about 50%), and even recovering animal motion behaviors, are realized, achieving an improved therapeutic effect without detectable adverse effects.
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Details
- Title: Subtitle
- Probiotic domestication and engineering enable one-shot treatment for bladder mucosal repair
- Creators
- Menglu Li - Jiangnan UniversityShengkai Jin - Jiangnan UniversityYichen Lu - Wuxi No.2 People's HospitalQingfei Sun - Hefei University of TechnologyYuwei Zhang - Nantong UniversityPeng Jiang - Jiangnan UniversitySha Zhu - Jiangnan UniversityYi Luo - University of IowaShan-Chao Zhao - Southern Medical UniversityChao Li - Hefei University of TechnologyNinghan Feng - Jiangnan University
- Resource Type
- Journal article
- Publication Details
- Biomaterials, Vol.318, 123123
- DOI
- 10.1016/j.biomaterials.2025.123123
- PMID
- 39893782
- NLM abbreviation
- Biomaterials
- ISSN
- 0142-9612
- eISSN
- 1878-5905
- Publisher
- Elsevier Ltd
- Grant note
- National Natural Science Foundation of China, China: 82302654, 82370777 Natural Science Foundation of Jiangsu Province, China: BK20230188 China Post- doctoral Science Foundation, Chian: 2024M752445 Anhui Provincial Natural Science Foundation, China: 2308085MB55 The "San Ming" Strategic Talent Project of Wuxi Municipal Health Commission, China: HB2023032 Science and Technology Research Project of Wuxi Science and Technology Bureau, China: K20221029
This work was supported by the National Natural Science Foundation of China, China (No. 82302654 and No. 82370777); Natural Science Foundation of Jiangsu Province, China (No. BK20230188); China Post- doctoral Science Foundation, Chian (No. 2024M752445); Anhui Provincial Natural Science Foundation, China (No. 2308085MB55); "San Ming" Strategic Talent Project of Wuxi Municipal Health Commission, China (No. HB2023032); Science and Technology Research Project of Wuxi Science and Technology Bureau, China (No. K20221029).
- Language
- English
- Electronic publication date
- 01/25/2025
- Date published
- 07/2025
- Academic Unit
- Urology
- Record Identifier
- 9984781274002771
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