Production and high throughput quantification of fucoxanthin and lipids in Tisochrysis lutea using single-cell fluorescence

Fengzheng Gao; Iago Teles; Narcis Ferrer-Ledo; Rene H. Wijffels; Maria J. Barbosa

doi:10.1016/j.biortech.2020.124104

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Production and high throughput quantification of fucoxanthin and lipids in Tisochrysis lutea using single-cell fluorescence

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Production and high throughput quantification of fucoxanthin and lipids in Tisochrysis lutea using single-cell fluorescence

Fengzheng Gao, Iago Teles, Narcis Ferrer-Ledo, Rene H. Wijffels and Maria J. Barbosa

Bioresource technology, Vol.318, pp.124104-124104

12/01/2020

DOI: 10.1016/j.biortech.2020.124104

PMID: 32942095

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Abstract

This work aimed to investigate the accumulation of fucoxanthin and lipids in Tisochrysis lutea during growth (N +) and nitrogen-starvation (N-) and to correlate these products with single-cell emissions using fluorescence activated cell sorting (FACS). Fucoxanthin content decreased 52.94% from N+ to N- in batch cultivation; increased 40.53% as dilution rate changed from 0.16 to 0.55 d(-1) in continuous cultivation. Total lipids (N-) were constant (similar to 250 mg/g), but the abundance of neutral lipids increased from 4.87% to 40.63%. Nile red can stain both polar and neutral lipids. However, in vivo, this differentiation is limited due to an overlapping of signals between 600 and 660 nm, caused by neutral lipids concentrations above 3.48% (W/W). Chlorophyll autofluorescence (720 nm) was reported for the first time as a proxy for fucoxanthin (R-2 = 0.90) and polar lipids (R-2 = 0.98). FACS can be used in high throughput quantification of pigments and lipids and to select and sort cells with high-fucoxanthin/lipids.

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Title: Subtitle: Production and high throughput quantification of fucoxanthin and lipids in Tisochrysis lutea using single-cell fluorescence
Creators: Fengzheng Gao - Wageningen University & Research
Iago Teles - Wageningen University & Research
Narcis Ferrer-Ledo - Wageningen University & Research
Rene H. Wijffels - Nord University
Maria J. Barbosa - Wageningen Univ, Bioproc Engn, AlgaePARC, POB 16, NL-6700 AA Wageningen, Netherlands
Resource Type: Journal article
Publication Details: Bioresource technology, Vol.318, pp.124104-124104
DOI: 10.1016/j.biortech.2020.124104
PMID: 32942095
NLM abbreviation: Bioresour Technol
ISSN: 0960-8524
eISSN: 1873-2976
Publisher: Elsevier
Number of pages: 8
Grant note: 745754 / Bio Based Industries Joint Undertaking under the European Union's Horizon 2020 research and innovation program
Language: English
Date published: 12/01/2020
Academic Unit: International Programs; Spanish and Portuguese
Record Identifier: 9984397910802771

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