Production and monitoring of biomass and fucoxanthin with brown microalgae under outdoor conditions

Fengzheng Gao; Marta Sá; Iago Teles Dominguez Cabanelas; René H. Wijffels; Maria J. Barbosa

doi:10.1002/bit.27657

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Production and monitoring of biomass and fucoxanthin with brown microalgae under outdoor conditions

Journal article

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Production and monitoring of biomass and fucoxanthin with brown microalgae under outdoor conditions

Fengzheng Gao, Marta Sá, Iago Teles Dominguez Cabanelas, René H. Wijffels and Maria J. Barbosa

Biotechnology and bioengineering, Vol.118(3), pp.1355-1365

03/2021

DOI: 10.1002/bit.27657

PMCID: PMC7986402

PMID: 33325031

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Abstract

The effect of light on biomass and fucoxanthin (Fx) productivities was studied in two microalgae, Tisochrysis lutea and Phaeodactylum tricornutum. High and low biomass concentrations (1.1 and 0.4 g L−1) were tested in outdoor pilot‐scale flat‐panel photobioreactors at semi‐continuous cultivation mode. Fluorescence spectroscopy coupled with chemometric modeling was used to develop prediction models for Fx content and for biomass concentration to be applied for both microalgae species. Prediction models showed high R2 for cell concentration (.93) and Fx content (.77). Biomass productivity was lower for high biomass concentration than low biomass concentration, for both microalgae (1.1 g L−1: 75.66 and 98.14 mg L−1 d−1, for T. lutea and P. tricornutum, respectively; 0.4 g L−1: 129.9 and 158.47 mg L−1 d−1, T. lutea and P. tricornutum). The same trend was observed in Fx productivity (1.1 g L−1: 1.14 and 1.41 mg L−1 d−1, T. lutea and P. tricornutum; 0.4 g L−1: 2.09 and 1.73 mg L−1 d−1, T. lutea and P. tricornutum). These results show that biomass and Fx productivities can be set by controlling biomass concentration under outdoor conditions and can be predicted using fluorescence spectroscopy. This monitoring tool opens new possibilities for online process control and optimization. Graphical Biomass and fucoxanthin outdoor production and monitoring with brown microalgae

chemometric modelling

fluorescence spectroscopy

fucoxanthin

Phaeodactylum tricornutum

Tisochrysis lutea

Details

Title: Subtitle: Production and monitoring of biomass and fucoxanthin with brown microalgae under outdoor conditions
Creators: Fengzheng Gao - Wageningen University & Research
Marta Sá - Wageningen University & Research
Iago Teles Dominguez Cabanelas - Wageningen University
René H. Wijffels - Nord University
Maria J. Barbosa - Wageningen University
Resource Type: Journal article
Publication Details: Biotechnology and bioengineering, Vol.118(3), pp.1355-1365
DOI: 10.1002/bit.27657
PMID: 33325031
PMCID: PMC7986402
NLM abbreviation: Biotechnol Bioeng
ISSN: 0006-3592
eISSN: 1097-0290
Number of pages: 11
Grant note: Horizon 2020 Framework Programme (745754)
Language: English
Date published: 03/2021
Academic Unit: International Programs; Spanish and Portuguese
Record Identifier: 9984397911702771

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