Journal article
Production of β-defensins by human airway epithelia
Proceedings of the National Academy of Sciences - PNAS, Vol.95(25), pp.14961-14966
12/08/1998
DOI: 10.1073/pnas.95.25.14961
PMCID: PMC24558
PMID: 9843998
Abstract
Human β-defensins (HBDs) are antimicrobial peptides that may play a role in mucosal defense. Diminished activity of these peptides has been implicated in the pathogenesis of cystic fibrosis (CF) lung disease. We show that HBD-1 and HBD-2 mRNAs are expressed in excised surface and submucosal gland epithelia from non-CF and CF patients. The pro-inflammatory cytokine interleukin-1β stimulated the expression of HBD-2 but not HBD-1 mRNA and peptide in primary cultures of airway epithelia. HBD-1 was found in bronchoalveolar lavage (BAL) fluid from normal volunteers, CF patients, and patients with inflammatory lung diseases, whereas HBD-2 was detected in BAL fluid from patients with CF or inflammatory lung diseases, but not in normal volunteers. Both HBD-1 and HBD-2 were found in BAL fluid in concentrations of several ng/ml, and both recombinant peptides showed salt-sensitive bactericidal activity. These data suggest that in the lung HBD-2 expression is induced by inflammation, whereas HBD-1 may serve as a defense in the absence of inflammation.
Details
- Title: Subtitle
- Production of β-defensins by human airway epithelia
- Creators
- Pradeep K Singh - Departments ofHong Peng Jia - Departments ofKerry Wiles - Departments ofJay Hesselberth - Departments ofLide Liu - Departments ofBarbara-Ann D Conway - Departments ofEverett P Greenberg - Departments ofErika V Valore - Departments ofMichael J Welsh - Departments ofTomas Ganz - Departments ofBrian F Tack - Departments ofPaul B McCray - Departments of
- Resource Type
- Journal article
- Publication Details
- Proceedings of the National Academy of Sciences - PNAS, Vol.95(25), pp.14961-14966
- DOI
- 10.1073/pnas.95.25.14961
- PMID
- 9843998
- PMCID
- PMC24558
- NLM abbreviation
- Proc Natl Acad Sci U S A
- ISSN
- 0027-8424
- eISSN
- 1091-6490
- Publisher
- National Academy of Sciences
- Language
- English
- Date published
- 12/08/1998
- Academic Unit
- Neurology; Molecular Physiology and Biophysics; Pulmonary, Critical Care, and Occupational Medicine; Microbiology and Immunology; Pulmonary Medicine; Stead Family Department of Pediatrics; Neurosurgery; Internal Medicine
- Record Identifier
- 9984020848002771
Metrics
24 Record Views